Abstract
A new technique for the production of hybrid strains of the cultivated mushroom Agaricus brunnescens is described. Homokaryons were recovered from regenerated protoplasts obtained from several heterokaryotic strains. A total of 16 novel hybrids were produced in 63 attempted crosses between paired homokaryons. Recovery of both homokaryons and hybrids was verified by analysis of restriction fragment length polymorphisms. Three of four hybrids fruited in small-scale tests, further confirming that the isolates were true hybrids. Colony morphology alone was found to be a poor indicator of hybrid status. In two instances, three homokaryons crossed successfully in all combinations, suggesting that there are at least three alleles at the putative mating-type locus. Crosses between homokaryons from commercial and wild-collected isolates indicated that these strains belong to the same biological species.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Castle A. J., Horgen P. A., Anderson J. B. Restriction fragment length polymorphisms in the mushrooms Agaricus brunnescens and Agaricus bitorquis. Appl Environ Microbiol. 1987 Apr;53(4):816–822. doi: 10.1128/aem.53.4.816-822.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EVANS H. J. Nuclear behaviour in the cultivated mushroom. Chromosoma. 1959;10(2):115–135. doi: 10.1007/BF00396566. [DOI] [PubMed] [Google Scholar]
- San Antonio J. P. A laboratory method to obtain fruit from cased grain spawn of the cultivated mushoom, Agaricus bisporus. Mycologia. 1971 Jan-Feb;63(1):16–21. [PubMed] [Google Scholar]
- Zolan M. E., Pukkila P. J. Inheritance of DNA methylation in Coprinus cinereus. Mol Cell Biol. 1986 Jan;6(1):195–200. doi: 10.1128/mcb.6.1.195. [DOI] [PMC free article] [PubMed] [Google Scholar]