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. 1988 Aug;54(8):2136–2139. doi: 10.1128/aem.54.8.2136-2139.1988

Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3.

B R Froseth 1, R E Herman 1, L L McKay 1
PMCID: PMC202817  PMID: 2845861

Abstract

The nisin resistance determinant and an origin of replication on pNP40, a plasmid of about 60 kilobases that is present in Streptococcus lactis subsp. diacetylactis DRC3, was cloned on a 7.6-kilobase EcoRI fragment. When self-ligated, this fragment existed as an independent replicon (pFM011) and contained a 2.6-kilobase EcoRI-XbaI fragment encoding nisin resistance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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