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. 1989 Jun;55(6):1649–1652. doi: 10.1128/aem.55.6.1649-1652.1989

Transformation of Bacillus cereus vegetative cells by electroporation.

B H Belliveau 1, J T Trevors 1
PMCID: PMC202922  PMID: 2504113

Abstract

Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with unpurified total plasmid DNA.

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Selected References

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  1. Allen S. P., Blaschek H. P. Electroporation-induced transformation of intact cells of Clostridium perfringens. Appl Environ Microbiol. 1988 Sep;54(9):2322–2324. doi: 10.1128/aem.54.9.2322-2324.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrews R. E., Jr, Faust R. M., Wabiko H., Raymond K. C., Bulla L. A., Jr The biotechnology of Bacillus thuringiensis. Crit Rev Biotechnol. 1987;6(2):163–232. doi: 10.3109/07388558709113596. [DOI] [PubMed] [Google Scholar]
  3. Aronson A. I., Beckman W., Dunn P. Bacillus thuringiensis and related insect pathogens. Microbiol Rev. 1986 Mar;50(1):1–24. doi: 10.1128/mr.50.1.1-24.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Aronson A. I., Beckman W. Transfer of chromosomal genes and plasmids in Bacillus thuringiensis. Appl Environ Microbiol. 1987 Jul;53(7):1525–1530. doi: 10.1128/aem.53.7.1525-1530.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Battisti L., Green B. D., Thorne C. B. Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. J Bacteriol. 1985 May;162(2):543–550. doi: 10.1128/jb.162.2.543-550.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bernhard K., Schrempf H., Goebel W. Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. J Bacteriol. 1978 Feb;133(2):897–903. doi: 10.1128/jb.133.2.897-903.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ehrlich S. D. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680–1682. doi: 10.1073/pnas.74.4.1680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Felkner I. C., Wyss O. A substance produced by competent Bacillus cereus 569 cells that affects transformability. Biochem Biophys Res Commun. 1964 May 22;16(1):94–99. doi: 10.1016/0006-291x(64)90217-7. [DOI] [PubMed] [Google Scholar]
  9. Fiedler S., Wirth R. Transformation of bacteria with plasmid DNA by electroporation. Anal Biochem. 1988 Apr;170(1):38–44. doi: 10.1016/0003-2697(88)90086-3. [DOI] [PubMed] [Google Scholar]
  10. González J. M., Jr, Brown B. J., Carlton B. C. Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxin among strains of B. thuringiensis and B. cereus. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6951–6955. doi: 10.1073/pnas.79.22.6951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. González J. M., Jr, Carlton B. C. Patterns of plasmid DNA in crystalliferous and acrystalliferous strains of Bacillus thuringiensis. Plasmid. 1980 Jan;3(1):92–98. doi: 10.1016/s0147-619x(80)90038-4. [DOI] [PubMed] [Google Scholar]
  12. Izaki K. Enzymatic reduction of mercurous and mercuric ions in Bacillus cereus. Can J Microbiol. 1981 Feb;27(2):192–197. doi: 10.1139/m81-030. [DOI] [PubMed] [Google Scholar]
  13. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Martin P. A., Lohr J. R., Dean D. H. Transformation of Bacillus thuringiensis protoplasts by plasmid deoxyribonucleic acid. J Bacteriol. 1981 Feb;145(2):980–983. doi: 10.1128/jb.145.2.980-983.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller J. F., Dower W. J., Tompkins L. S. High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc Natl Acad Sci U S A. 1988 Feb;85(3):856–860. doi: 10.1073/pnas.85.3.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neumann E., Schaefer-Ridder M., Wang Y., Hofschneider P. H. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1982;1(7):841–845. doi: 10.1002/j.1460-2075.1982.tb01257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Powell Ian B., Achen Marc G., Hillier Alan J., Davidson Barrie E. A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation. Appl Environ Microbiol. 1988 Mar;54(3):655–660. doi: 10.1128/aem.54.3.655-660.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shigekawa K., Dower W. J. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. Biotechniques. 1988 Sep;6(8):742–751. [PubMed] [Google Scholar]
  19. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yelton D. B., Thorne C. B. Comparison of Bacillus cereus bacteriophages CP-51 and CP-53. J Virol. 1971 Aug;8(2):242–253. doi: 10.1128/jvi.8.2.242-253.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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