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Giα1 inhibits the activity of different forms of ACV. Supernatants of cell lysates containing soluble forms of ACV or membranes from Sf9 cells expressing full-length ACV were assayed for AC activity in the presence of forskolin (100 μM) in the presence or absence of Giα1 (1 μM). Forskolin-stimulated activity for all forms of ACV is presented as 100% to facilitate comparisons. The data are from three determinations (mean ± SEM).
