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. 1989 Aug;55(8):2025–2029. doi: 10.1128/aem.55.8.2025-2029.1989

Specific Dot-Immunobinding Assay for Detection and Enumeration of Thiobacillus ferrooxidans

Renato Arredondo 1, Carlos A Jerez 1,*
PMCID: PMC202997  PMID: 16347993

Abstract

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in 125I-labeled protein A or 125I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. Since a minimum of 103 cells per dot could be detected, the method offers the possibility of simultaneous processing of numerous samples in a short time to monitor the levels of T. ferrooxidans in bioleaching operations.

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Selected References

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