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. 1989 Aug;55(8):2041–2045. doi: 10.1128/aem.55.8.2041-2045.1989

Evaluation of media for monitoring fecal streptococci in seawater.

Y Yoshpe-Purer 1
PMCID: PMC203000  PMID: 2782876

Abstract

The selectivity of KF streptococcus agar (KF) for monitoring fecal streptococci (FS) in seawater was examined in 234 samples of Mediterranean water and compared with the selectivity of M-Enterococcus agar (M-Ent) for 124 samples and with bile-esculin-azide agar (BEA) for 17 samples. KF was found to be unsuitable for marine water because Vibrio alginolyticus and other gram-negative bacilli indigenous to this environment grew well on it and produced red colonies identical to those of FS. In 26% of samples, some with high counts of red colonies on the membrane filters (MF), there were no streptococci, only gram-negative bacilli and staphylococci, and in an additional 23.1% the streptococci constituted less than 50% of the "typical" red colonies on the MF. V. alginolyticus also produced FS-like colonies on MF incubated on BEA but was not isolated from MF incubated on M-Ent. Although staphylococci grew and produced FS-like colonies on all three media, M-Ent was the most selective since no gram-negative bacilli were isolated from MF incubated on it.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodsky M. H., Schiemann D. A. Evaluation of Pfizer selective enterococcus and KF media for recovery of fecal streptococci from water by membrane filtration. Appl Environ Microbiol. 1976 May;31(5):695–699. doi: 10.1128/aem.31.5.695-699.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buck J. D. Occurrence of false-positive most probable number tests for fecal streptococci in marine waters. Appl Microbiol. 1969 Oct;18(4):562–565. doi: 10.1128/am.18.4.562-565.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buck J. D. Selective detection of enterococci in marine waters. Am J Public Health. 1972 Mar;62(3):419–421. doi: 10.2105/ajph.62.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daoust R. A., Litsky W. Pfizer selective enterococcus agar overlay method for the enumeration of fecal streptococci by membrane filtration. Appl Microbiol. 1975 May;29(5):584–589. doi: 10.1128/am.29.5.584-589.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donnelly L. S., Hartman P. A. Gentamicin-based medium for the isolation of group D streptococci and application of the medium to water analysis. Appl Environ Microbiol. 1978 Mar;35(3):576–581. doi: 10.1128/aem.35.3.576-581.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R., Moody M. D. Presumptive identification of group D streptococci: the bile-esculin test. Appl Microbiol. 1970 Aug;20(2):245–250. doi: 10.1128/am.20.2.245-250.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Facklam R. R. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. doi: 10.1128/am.23.6.1131-1139.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Isenberg H. D., Goldberg D., Sampson J. Laboratory studies with a selective Enterococcus medium. Appl Microbiol. 1970 Sep;20(3):433–436. doi: 10.1128/am.20.3.433-436.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KENNER B. A., CLARK H. F., KABLER P. W. Fecal Streptococci. I. Cultivation and enumeration of Streptococci in surface waters. Appl Microbiol. 1961 Jan;9:15–20. doi: 10.1128/am.9.1.15-20.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levin M. A., Fischer J. R., Cabelli V. J. Membrane filter technique for enumeration of enterococci in marine waters. Appl Microbiol. 1975 Jul;30(1):66–71. doi: 10.1128/am.30.1.66-71.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pavlova M. T., Brezenski F. T., Litsky W. Evaluation of various media for isolation, enumeration and identification of fecal streptococci from natural sources. Health Lab Sci. 1972 Oct;9(4):289–298. [PubMed] [Google Scholar]
  12. SLANETZ L. W., BARTLEY C. H. DETECTION AND SANITARY SIGNIFICANCE OF FECAL STREPTOCOCCI IN WATER. Am J Public Health Nations Health. 1964 Apr;54:609–614. doi: 10.2105/ajph.54.4.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SLANETZ L. W., BARTLEY C. H. Numbers of enterococci in water, sewage, and feces determined by the membrane filter technique with an improved medium. J Bacteriol. 1957 Nov;74(5):591–595. doi: 10.1128/jb.74.5.591-595.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sabbaj J., Sutter V. L., Finegold S. M. Comparison of selective media for isolation of presumptive group D streptococci from human feces. Appl Microbiol. 1971 Dec;22(6):1008–1011. doi: 10.1128/am.22.6.1008-1011.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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