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. 1989 Aug;55(8):2056–2060. doi: 10.1128/aem.55.8.2056-2060.1989

Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.

C Verreth 1, B Cammue 1, P Swinnen 1, D Crombez 1, A Michielsen 1, K Michiels 1, A Van Gool 1, J Vanderleyden 1
PMCID: PMC203002  PMID: 2675764

Abstract

The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase gene was found to be highly amplified in the Azospirillum genome, scattered in the chromosomal as well as in the plasmidic DNA.

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Selected References

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