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. 1989 Aug;55(8):2095–2097. doi: 10.1128/aem.55.8.2095-2097.1989

Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.

E E Bates 1, H J Gilbert 1, G P Hazlewood 1, J Huckle 1, J I Laurie 1, S P Mann 1
PMCID: PMC203011  PMID: 2782880

Abstract

Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two other plasmids, pGK12 and pSA3, were transformed into L. plantarum, and the stability of each plasmid was evaluated.

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Selected References

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