Abstract
Constraints created by immobilization conditions modified the physiological behavior and morphological characteristics of Gibberella fujikuroi mycelia in comparison with their development in free-cell conditions. G. fujikuroi mycelia were immobilized in different support matrices (polyurethane, carrageenan, and alginate) and showed a variety of reactions in response to the different microenvironmental factors encountered during and after immobilization. The best support with respect to gibberellic acid yield and biocatalyst stability was found to be an alginate with a high degree of polymerization. The most visible effects of immobilization included changes in growth development, morphological appearance, metabolite production, mycelial pigmentation, mycelial viability under starvation conditions, and induction of resting forms when previously immobilized mycelia were subcultured.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Avalos J., Casadesús J., Cerdá-Olmedo E. Gibberella fujikuroi mutants obtained with UV radiation and N-methyl-N'-nitro-N-nitrosoguanidine. Appl Environ Microbiol. 1985 Jan;49(1):187–191. doi: 10.1128/aem.49.1.187-191.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deo Y. M., Costerton J. W., Gaucher G. M. Examination of immobilized fungal cells by phase-contrast and scanning electron microscopy. Can J Microbiol. 1983 Dec;29(12):1642–1649. doi: 10.1139/m83-251. [DOI] [PubMed] [Google Scholar]
- Gaucher G. M., Behie L. A. Cell immobilization in the production of patulin and penicillin by Penicillium urticae and Penicillium chrysogenum. Methods Enzymol. 1987;136:329–342. doi: 10.1016/s0076-6879(87)36032-x. [DOI] [PubMed] [Google Scholar]
- Gelderblom W. C., Jaskiewicz K., Marasas W. F., Thiel P. G., Horak R. M., Vleggaar R., Kriek N. P. Fumonisins--novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Appl Environ Microbiol. 1988 Jul;54(7):1806–1811. doi: 10.1128/aem.54.7.1806-1811.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kjaer D., Kjaer A., Pedersen C., Bu'lock J. D., Smith J. R. Bikaverin and norbikaverin, benzoxanthentrione pigments of Gibberella fujikuroi. J Chem Soc Perkin 1. 1971;16:2792–2797. doi: 10.1039/j39710002792. [DOI] [PubMed] [Google Scholar]
- Schmid J., Harold F. M. Dual roles for calcium ions in apical growth of Neurospora crassa. J Gen Microbiol. 1988 Sep;134(9):2623–2631. doi: 10.1099/00221287-134-9-2623. [DOI] [PubMed] [Google Scholar]