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. 1989 Oct;55(10):2729–2731. doi: 10.1128/aem.55.10.2729-2731.1989

Electron Spin Resonance Analysis of the Effect of Butanol on the Membrane Fluidity of Intact Cells of Clostridium acetobutylicum

Shirley H Baer 1, Dennis L Bryant 1, Hans P Blaschek 1,*
PMCID: PMC203153  PMID: 16348040

Abstract

Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical fluidizing effect of high (1.5%) butanol challenge by reducing its membrane fluidity at 12 and 30 h. The wild-type 824 strain was unable to so respond when challenged at the 1.5% level.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baer S. H., Blaschek H. P., Smith T. L. Effect of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium acetobutylicum. Appl Environ Microbiol. 1987 Dec;53(12):2854–2861. doi: 10.1128/aem.53.12.2854-2861.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Finne G., Matches J. R. Spin-labeling studies on the lipids of psychrophilic, psychrotrophic, and mesophilic clostridia. J Bacteriol. 1976 Jan;125(1):211–219. doi: 10.1128/jb.125.1.211-219.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ingram L. O., Buttke T. M. Effects of alcohols on micro-organisms. Adv Microb Physiol. 1984;25:253–300. doi: 10.1016/s0065-2911(08)60294-5. [DOI] [PubMed] [Google Scholar]
  4. Lenaz G., Bertoli E., Curatola G., Mazzanti L., Bigi A. Lipid protein interactions in mitochondria. Spin and fluorescence probe studies on the effect of n-alkanols on phospholipid vesicles and mitochondrial membranes. Arch Biochem Biophys. 1976 Jan;172(1):278–288. doi: 10.1016/0003-9861(76)90077-1. [DOI] [PubMed] [Google Scholar]
  5. Nettleton D. O., Morse P. D., 2nd, Dobrucki J. W., Swartz H. M., Dodd N. J. Distribution of 5-doxylstearic acid in the membranes of mammalian cells. Biochim Biophys Acta. 1988 Oct 6;944(2):315–320. doi: 10.1016/0005-2736(88)90446-4. [DOI] [PubMed] [Google Scholar]
  6. Vollherbst-Schneck K., Sands J. A., Montenecourt B. S. Effect of butanol on lipid composition and fluidity of Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1984 Jan;47(1):193–194. doi: 10.1128/aem.47.1.193-194.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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