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. 1989 Nov;55(11):2985–2989. doi: 10.1128/aem.55.11.2985-2989.1989

Response of Plant-Colonizing Pseudomonads to Hydrogen Peroxide †

J Katsuwon 1, A J Anderson 1,*
PMCID: PMC203201  PMID: 16348058

Abstract

Colonization of plant root surfaces by Pseudomonas putida may require mechanisms that protect this bacterium against superoxide anion and hydrogen peroxide produced by the root. Catalase and superoxide dismutase may be important in this bacterial defense system. Stationary-phase cells of P. putida were not killed by hydrogen peroxide (H2O2) at concentrations up to 10 mM, and extracts from these cells possessed three isozymic bands (A, B, and C) of catalase activity in native polyacrylamide gel electrophoresis. Logarithmic-phase cells exposed directly to hydrogen peroxide concentrations above 1 mM were killed. Extracts of logarithmic-phase cells displayed only band A catalase activity. Protection against 5 mM H2O2 was apparent after previous exposure of the logarithmic-phase cells to nonlethal concentrations (30 to 300 μM) of H2O2. Extracts of these protected cells possessed enhanced catalase activity of band A and small amounts of bands B and C. A single form of superoxide dismutase and isoforms of catalase were apparent in extracts from a foliar intercellular pathogen, Pseudomonas syringae pv. phaseolicola. The mobilities of these P. syringae enzymes were distinct from those of enzymes in P. putida extracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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