Abstract
A procedure for plating Sulfolobus acidocaldarius using Gelrite as the gelling agent is presented. The technique uses a supporting gel of 0.8% (wt/vol) Gelrite and an overlay soft gel of 0.4% (wt/vol) Gelrite, in which the colonies are grown. The plating efficiency was essentially 100%.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Deming J. W., Baross J. A. Solid Medium for Culturing Black Smoker Bacteria at Temperatures to 120 degrees C. Appl Environ Microbiol. 1986 Feb;51(2):238–243. doi: 10.1128/aem.51.2.238-243.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lin C. C., Casida L. E. GELRITE as a Gelling Agent in Media for the Growth of Thermophilic Microorganisms. Appl Environ Microbiol. 1984 Feb;47(2):427–429. doi: 10.1128/aem.47.2.427-429.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol. 1959 May;77(5):642–647. doi: 10.1128/jb.77.5.642-647.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]