Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1977 Nov;89(2):351–366.

Profile of intracytoplasmic lysozyme in normal tissues, myeloproliferative disorders, hairy cell leukemia, and other pathologic processes. An immunoperoxidase study of paraffin sections and smears.

G S Pinkus, J W Said
PMCID: PMC2032246  PMID: 335890

Abstract

Intracytoplasmic lysozyme (muramidase) may be readily identified in paraffin sections of tissues fixed in formalin or Zenker's acetic acid and in smears of peripheral blood or bone marrow using an immunoperoxidase technique. Sites of intracellular lysozyme in normal human tissues and in various specimens from patients with myeloproliferative and lymphoproliferative disorders, hairy cell leukemia, granulomatous diseases, toxoplasmic lymphadenitis, and other pathologic processes were defined by this method. Intracellular lysozyme was demonstrated in mature and immature neutrophilic and eosinophilic myeloid cells, in monocytic cells, and in some types of histiocytes and had a limited distribution in normal tissues. The neoplastic cells of hairy cell leukemia were devoid of intracytoplasmic lysozyme. Identification of intracellular lysozyme, as determined by the immunoperoxidase technique, was compared with various cytochemical methods, particularly chloroacetate esterase and alpha-naphthyl butyrate esterase studies, for detection and characterization of myeloid cells, monocytes, and histiocytes.

Full text

PDF
351

Images in this article

366Figure 7
on p.366
366Figure 8
on p.366
363Figure 1
on p.363
363Figure 2
on p.363
363Figure 3
on p.363
364Figure 4
on p.364
364Figure 5
on p.364
365Figure 6
on p.365

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asamer H., Schmalzl F., Braunsteiner H. Der immunzytologische Lysozymnachweis in menschlichen Blutzellen. Acta Haematol. 1969;41(1):49–54. doi: 10.1159/000208830. [DOI] [PubMed] [Google Scholar]
  2. Asamer H., Schmalzl F., Braunsteiner H. Immunocytological demonstration of lysozyme (muramidase) in human leukaemic cells. Br J Haematol. 1971 Jun;20(6):571–574. doi: 10.1111/j.1365-2141.1971.tb00795.x. [DOI] [PubMed] [Google Scholar]
  3. Briggs R. S., Perillie P. E., Finch S. C. Lysozyme in bone marrow and peripheral blood cells. J Histochem Cytochem. 1966 Feb;14(2):167–170. doi: 10.1177/14.2.167. [DOI] [PubMed] [Google Scholar]
  4. Burns C. P. Letter: Serum-muramidase in leukaemic reticuloendotheliosis. Lancet. 1974 Oct 19;2(7886):964–964. doi: 10.1016/s0140-6736(74)91184-2. [DOI] [PubMed] [Google Scholar]
  5. Burns C. P., Maca R. D., Hoak J. C. Biochemical, morphological, and immunological observations of leukemic reticuloendotheliosis. Cancer Res. 1973 Jul;33(7):1615–1624. [PubMed] [Google Scholar]
  6. Catovsky D., Galton D. A., Griffin C. The significance of lysozyme estimations in acute myeloid and chronic monocytic leukaemia. Br J Haematol. 1971 Nov;21(5):565–580. doi: 10.1111/j.1365-2141.1971.tb02718.x. [DOI] [PubMed] [Google Scholar]
  7. Catovsky D. Hairy-cell leukaemia and prolymphocytic leukaemia. Clin Haematol. 1977 Feb;6(1):245–268. [PubMed] [Google Scholar]
  8. FLANAGAN P., LIONETTI F. Lysozyme distribution in blood. Blood. 1955 May;10(5):497–501. [PubMed] [Google Scholar]
  9. Falchuk K. R., Perrotto J. L., Isselbacher K. J. Serum lysozyme in Crohn's disease and ulcerative colitis. N Engl J Med. 1975 Feb 20;292(8):395–397. doi: 10.1056/NEJM197502202920805. [DOI] [PubMed] [Google Scholar]
  10. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  11. Hansen N. E. Lysozyme in Haematology pathophysiology and clinical use. Scand J Haematol. 1975 May;14(3):160–165. [PubMed] [Google Scholar]
  12. Jaffe E. S., Shevach E. M., Frank M. M., Green I. Leukemic reticuloendotheliosis: presence of a receptor for cytophilic antibody. Am J Med. 1974 Jul;57(1):108–114. doi: 10.1016/0002-9343(74)90775-x. [DOI] [PubMed] [Google Scholar]
  13. Johansson B. G., Malmquist J. Quantitative immunochemical determination of lysoqyme (muramidase) in serum and urine. Scand J Clin Lab Invest. 1971 May;27(3):255–261. doi: 10.3109/00365517109080216. [DOI] [PubMed] [Google Scholar]
  14. Karle H., Hansen N. E., Killmann S. A. Intracellular lysozyme in mature neutrophils and blast cells in acute leukemia. Blood. 1974 Aug;44(2):247–255. [PubMed] [Google Scholar]
  15. LITWACK G. Photometric determination of lysozyme activity. Proc Soc Exp Biol Med. 1955 Jul;89(3):401–403. doi: 10.3181/00379727-89-21824. [DOI] [PubMed] [Google Scholar]
  16. Levi J. A., Speden J. B., Vincent P. C., Gunz F. W. Studies on muramidase in hematologic disorders. I. Serum muramidase and serum lactic dehydrogenase in leukemia. Cancer. 1973 Apr;31(4):939–947. doi: 10.1002/1097-0142(197304)31:4<939::aid-cncr2820310427>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  17. Li C. Y., Yam L. T., Crosby W. H. Histochemical characterization of cellular and structural elements of the human spleen. J Histochem Cytochem. 1972 Dec;20(12):1049–1058. doi: 10.1177/20.12.1049. [DOI] [PubMed] [Google Scholar]
  18. Lukes R. J., Collins R. D. Immunologic characterization of human malignant lymphomas. Cancer. 1974 Oct;34(4 Suppl):suppl–suppl:1503. doi: 10.1002/1097-0142(197410)34:8+<1488::aid-cncr2820340822>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  19. MOLONEY W. C., MCPHERSON K., FLIEGELMAN L. Esterase activity in leukocytes demonstrated by the use of naphthol AS-D chloroacetate substrate. J Histochem Cytochem. 1960 May;8:200–207. doi: 10.1177/8.3.200. [DOI] [PubMed] [Google Scholar]
  20. Mason D. Y., Taylor C. R. The distribution of muramidase (lysozyme) in human tissues. J Clin Pathol. 1975 Feb;28(2):124–132. doi: 10.1136/jcp.28.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perillie P. E., Khan K., Finch S. C. Serum lysozyme in pulmonary tuberculosis. Am J Med Sci. 1973 Apr;265(4):297–302. doi: 10.1097/00000441-197304000-00005. [DOI] [PubMed] [Google Scholar]
  23. Pinkus G. S., Said J. W. Specific identification of intracellular immunoglobulin in paraffin sections of multiple myeloma and macroglobulinemia using an immunoperoxidase technique. Am J Pathol. 1977 Apr;87(1):47–57. [PMC free article] [PubMed] [Google Scholar]
  24. Schnitzer B., Kass L. Hairy-cell leukemia. A clinicopathologic and ultrastructural study. Am J Clin Pathol. 1974 Feb;61(2):176–187. doi: 10.1093/ajcp/61.2.176. [DOI] [PubMed] [Google Scholar]
  25. Syrén E., Raeste A. M. Identification of blood monocytes by demonstration of lysozyme and peroxidase activity. Acta Haematol. 1971;45(1):29–35. doi: 10.1159/000208604. [DOI] [PubMed] [Google Scholar]
  26. Taylor C. R. An immunohistological study of follicular lymphoma, reticulum cell sarcoma and Hodgkin's disease. Eur J Cancer. 1976 Jan;12(1):61–75. doi: 10.1016/0014-2964(76)90125-0. [DOI] [PubMed] [Google Scholar]
  27. Taylor C. R. The nature of Reed-Sternberg cells and other malignant "reticulum" cells. Lancet. 1974 Oct 5;2(7884):802–807. doi: 10.1016/s0140-6736(74)91071-x. [DOI] [PubMed] [Google Scholar]
  28. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES