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. 1989 Dec;55(12):3085–3090. doi: 10.1128/aem.55.12.3085-3090.1989

Action of a Fluoranthene-Utilizing Bacterial Community on Polycyclic Aromatic Hydrocarbon Components of Creosote

J G Mueller 1,*, P J Chapman 1, P H Pritchard 1
PMCID: PMC203227  PMID: 16348069

Abstract

Cultures enriched by serial transfer through a mineral salts medium containing fluoranthene were used to establish a stable, seven-member bacterial community from a sandy soil highly contaminated with coal tar creosote. This community exhibited an ability to utilize fluoranthene as the sole carbon source for growth, as demonstrated by increases in protein concentration and changes in absorption spectra when grown on fluoranthene in liquid culture. Biotransformation of other polycyclic aromatic hydrocarbons (PAHs) was verified by demonstrating their disappearance from an artificial PAH mixture by capillary gas chromatography. When grown on fluoranthene as the sole carbon source and subsequently exposed to fluoranthene plus 16 additional PAHs typical of those found in creosote, this community transformed all PAHs present in this defined mixture. After 3 days of incubation, 13 of the original 17 PAH components were degraded to levels below the limit of detection (10 ng/liter). Continued incubation resulted in extensive degradation of the remaining four compounds. The ability of this community to utilize a high-molecular-weight PAH as the sole carbon source, in conjunction with its ability to transform a diverse array of PAHs, suggests that it may be of value in the bioremediation of environments contaminated with PAHs, such as those impacted by creosote.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnsley E. A. The bacterial degradation of fluoranthene and benzo[alpyrene. Can J Microbiol. 1975 Jul;21(7):1004–1008. doi: 10.1139/m75-148. [DOI] [PubMed] [Google Scholar]
  2. Bumpus J. A. Biodegradation of polycyclic hydrocarbons by Phanerochaete chrysosporium. Appl Environ Microbiol. 1989 Jan;55(1):154–158. doi: 10.1128/aem.55.1.154-158.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cerniglia C. E., Gibson D. T. Oxidation of benzo[a]pyrene by the filamentous fungus Cunninghamella elegans. J Biol Chem. 1979 Dec 10;254(23):12174–12180. [PubMed] [Google Scholar]
  4. Cerniglia C. E. Microbial metabolism of polycyclic aromatic hydrocarbons. Adv Appl Microbiol. 1984;30:31–71. doi: 10.1016/s0065-2164(08)70052-2. [DOI] [PubMed] [Google Scholar]
  5. Cerniglia C. E., Yang S. K. Stereoselective metabolism of anthracene and phenanthrene by the fungus Cunninghamella elegans. Appl Environ Microbiol. 1984 Jan;47(1):119–124. doi: 10.1128/aem.47.1.119-124.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies J. I., Evans W. C. Oxidative metabolism of naphthalene by soil pseudomonads. The ring-fission mechanism. Biochem J. 1964 May;91(2):251–261. doi: 10.1042/bj0910251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furukawa K., Miyazaki T. Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. J Bacteriol. 1986 May;166(2):392–398. doi: 10.1128/jb.166.2.392-398.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gibson D. T., Mahadevan V., Jerina D. M., Yogi H., Yeh H. J. Oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium. Science. 1975 Jul 25;189(4199):295–297. doi: 10.1126/science.1145203. [DOI] [PubMed] [Google Scholar]
  9. Gibson D. T., Roberts R. L., Wells M. C., Kobal V. M. Oxidation of biphenyl by a Beijerinckia species. Biochem Biophys Res Commun. 1973 Jan 23;50(2):211–219. doi: 10.1016/0006-291x(73)90828-0. [DOI] [PubMed] [Google Scholar]
  10. Guerin W. F., Jones G. E. Mineralization of phenanthrene by a Mycobacterium sp. Appl Environ Microbiol. 1988 Apr;54(4):937–944. doi: 10.1128/aem.54.4.937-944.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heitkamp M. A., Cerniglia C. E. Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. Appl Environ Microbiol. 1988 Jun;54(6):1612–1614. doi: 10.1128/aem.54.6.1612-1614.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Heitkamp M. A., Franklin W., Cerniglia C. E. Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium. Appl Environ Microbiol. 1988 Oct;54(10):2549–2555. doi: 10.1128/aem.54.10.2549-2555.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heitkamp M. A., Freeman J. P., Miller D. W., Cerniglia C. E. Pyrene degradation by a Mycobacterium sp.: identification of ring oxidation and ring fission products. Appl Environ Microbiol. 1988 Oct;54(10):2556–2565. doi: 10.1128/aem.54.10.2556-2565.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herbes S. E., Schwall L. R. Microbial transformation of polycyclic aromatic hydrocarbons in pristine and petroleum-contaminated sediments. Appl Environ Microbiol. 1978 Feb;35(2):306–316. doi: 10.1128/aem.35.2.306-316.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jeffrey A. M., Yeh H. J., Jerina D. M., Patel T. R., Davey J. F., Gibson D. T. Initial reactions in the oxidation of naphthalene by Pseudomonas putida. Biochemistry. 1975 Feb 11;14(3):575–584. doi: 10.1021/bi00674a018. [DOI] [PubMed] [Google Scholar]
  16. Jerina D. M., Selander H., Yagi H., Wells M. C., Davey J. F., Mahadevan V., Gibson D. T. Dihydrodiols from anthracene and phenanthrene. J Am Chem Soc. 1976 Sep 15;98(19):5988–5996. doi: 10.1021/ja00435a035. [DOI] [PubMed] [Google Scholar]
  17. Mahaffey W. R., Gibson D. T., Cerniglia C. E. Bacterial oxidation of chemical carcinogens: formation of polycyclic aromatic acids from benz[a]anthracene. Appl Environ Microbiol. 1988 Oct;54(10):2415–2423. doi: 10.1128/aem.54.10.2415-2423.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Morgan P., Watkinson R. J. Hydrocarbon degradation in soils and methods for soil biotreatment. Crit Rev Biotechnol. 1989;8(4):305–333. doi: 10.3109/07388558909148196. [DOI] [PubMed] [Google Scholar]

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