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. 1975 Oct;81(1):1–14.

Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B.

L S Hsu, E L Becker
PMCID: PMC2032297  PMID: 1180327

Abstract

Incubation of rabbit neutrophils with various chemotactic factors causes an expansion of their volume. The expansion shows a high correlation with the chemotactic responsiveness of these cells, requires metabolic energy, and is independent of the presence of divalent cations. Cytochalasin B causes a decrease in the volume of the neutrophil. This decrease also requires metabolic energy and is independent of divalent cations. In the presence of cytochalasin B, the chemotactic factor, instead of acting to expand cell volume, induces a further contraction of the cell; this decrease requires Ca2+ in the external medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker E. L., Showell H. J., Henson P. M., Hsu L. S. The ability of chemotactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness. J Immunol. 1974 Jun;112(6):2047–2054. [PubMed] [Google Scholar]
  2. Becker E. L., Showell H. J. The effect of Ca2+ and Mg2+ on the chemotactic responsiveness and spontaneous motility of rabbit polymorphonuclear leukocytes. Z Immunitatsforsch Exp Klin Immunol. 1972 Jun;143(5):466–476. [PubMed] [Google Scholar]
  3. Dunham P. B., Goldstein I. M., Weissmann G. Potassium and amino acid transport in human leukocytes exposed to phagocytic stimuli. J Cell Biol. 1974 Oct;63(1):215–226. doi: 10.1083/jcb.63.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fay F. S., Delise C. M. Contraction of isolated smooth-muscle cells--structural changes. Proc Natl Acad Sci U S A. 1973 Mar;70(3):641–645. doi: 10.1073/pnas.70.3.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gallin J. I., Rosenthal A. S. The regulatory role of divalent cations in human granulocyte chemotaxis. Evidence for an association between calcium exchanges and microtubule assembly. J Cell Biol. 1974 Sep;62(3):594–609. doi: 10.1083/jcb.62.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldstein I., Hoffstein S., Gallin J., Weissmann G. Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2916–2920. doi: 10.1073/pnas.70.10.2916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hsu L. S., Becker E. L. Contraction and volume changes of glycerol treated rabbit polymorphonuclear leukocytes induced by ATP and Ca 2 ion. Proc Soc Exp Biol Med. 1974 Jun;146(2):453–457. doi: 10.3181/00379727-146-38124. [DOI] [PubMed] [Google Scholar]
  8. Hsu L. S., Becker E. L. Volume decrease of glycerinated polymorphoneclear leukocytes induced by ATP and Ca-2. Exp Cell Res. 1975 Mar 15;91(2):469–473. doi: 10.1016/0014-4827(75)90131-7. [DOI] [PubMed] [Google Scholar]
  9. Koza E. P., Wright T. E., Becker E. L. Lysosomal enzyme secretion and volume contraction induced in neutrophils by cytochalasin B, chemotactic factor and A23187. Proc Soc Exp Biol Med. 1975 Jun;149(2):476–479. doi: 10.3181/00379727-149-38831. [DOI] [PubMed] [Google Scholar]
  10. MARSH B. B. The effects of adenosine triphosphate on the fibre volume of a muscle homogenate. Biochim Biophys Acta. 1952 Sep;9(3):247–260. doi: 10.1016/0006-3002(52)90159-5. [DOI] [PubMed] [Google Scholar]
  11. Miranda A. F., Godman G. C., Deitch A. D., Tanenbaum S. W. Action of cytochalasin D on cells of established lines. I. Early events. J Cell Biol. 1974 May;61(2):481–500. doi: 10.1083/jcb.61.2.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Palek J., Curby W. A., Lionetti F. J. Relation of Ca plus plus-activated ATPase to Ca plus plus-linked shrinkage of human red cell ghosts. Am J Physiol. 1971 Apr;220(4):1028–1032. doi: 10.1152/ajplegacy.1971.220.4.1028. [DOI] [PubMed] [Google Scholar]
  13. Schiffmann E., Showell H. V., Corcoran B. A., Ward P. A., Smith E., Becker E. L. The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli. J Immunol. 1975 Jun;114(6):1831–1837. [PubMed] [Google Scholar]

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