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. 1989 Dec;55(12):3119–3123. doi: 10.1128/aem.55.12.3119-3123.1989

High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media

Helge Holo 1,†,*, Ingolf F Nes 1
PMCID: PMC203233  PMID: 16348073

Abstract

An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be stored at −85°C without loss of competence. With Lactococcus lactis subsp. cremoris BC101, a transformation frequency of 5.7 × 107 transformants per μg of pIL253 DNA was obtained, which represents 5% of the surviving cells. All the lactococcal strains tested could be transformed by the present method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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