Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1989 Dec;55(12):3250–3252. doi: 10.1128/aem.55.12.3250-3252.1989

Development of enterobacterium-specific oligonucleotide probes based on the surface-exposed regions of outer membrane proteins.

G Spierings 1, H Hofstra 1, J Huis in'T Veld 1, W Hoekstra 1, J Tommassen 1
PMCID: PMC203259  PMID: 2619311

Abstract

Outer membrane proteins of members of the family Enterobacteriaceae consist of conserved membrane-spanning segments and hypervariable, surface-exposed regions. We demonstrate that the hypervariable DNA segments corresponding to the surface-exposed regions of these proteins can be used to develop specific DNA probes for the identification of members of the family Enterobacteriaceae.

Full text

PDF
3250

Images in this article

3251Image
on p.3251
3251Image
on p.3251

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun G., Cole S. T. DNA sequence analysis of the Serratia marcescens ompA gene: implications for the organisation of an enterobacterial outer membrane protein. Mol Gen Genet. 1984;195(1-2):321–328. doi: 10.1007/BF00332766. [DOI] [PubMed] [Google Scholar]
  2. Carter P. J., Winter G., Wilkinson A. J., Fersht A. R. The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus). Cell. 1984 Oct;38(3):835–840. doi: 10.1016/0092-8674(84)90278-2. [DOI] [PubMed] [Google Scholar]
  3. Hofstra H., Dankert J. Major outer membrane proteins: common antigens in enterobacteriaceae species. J Gen Microbiol. 1980 Jul;119(1):123–131. doi: 10.1099/00221287-119-1-123. [DOI] [PubMed] [Google Scholar]
  4. Mizuno T., Chou M. Y., Inouye M. A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene. J Biol Chem. 1983 Jun 10;258(11):6932–6940. [PubMed] [Google Scholar]
  5. Morona R., Klose M., Henning U. Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins. J Bacteriol. 1984 Aug;159(2):570–578. doi: 10.1128/jb.159.2.570-578.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Overbeeke N., Lugtenberg B. Expression of outer membrane protein e of Escherichia coli K12 by phosphate limitation. FEBS Lett. 1980 Apr 7;112(2):229–232. doi: 10.1016/0014-5793(80)80186-4. [DOI] [PubMed] [Google Scholar]
  7. Overbeeke N., Lugtenberg B. Major outer membrane proteins of Escherichia coli strains of human origin. J Gen Microbiol. 1980 Dec;121(2):373–380. doi: 10.1099/00221287-121-2-373. [DOI] [PubMed] [Google Scholar]
  8. Sato T., Yura T. Chromosomal location and expression of the structural gene for major outer membrane protein Ia of Escherichia coli K-12 and of the homologous gene of Salmonella typhimurium. J Bacteriol. 1979 Aug;139(2):468–477. doi: 10.1128/jb.139.2.468-477.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tommassen J., Overduin P., Lugtenberg B., Bergmans H. Cloning of phoE, the structural gene for the Escherichia coli phosphate limitation-inducible outer membrane pore protein. J Bacteriol. 1982 Feb;149(2):668–672. doi: 10.1128/jb.149.2.668-672.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tommassen J., van Tol H., Lugtenberg B. The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence. EMBO J. 1983;2(8):1275–1279. doi: 10.1002/j.1460-2075.1983.tb01581.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Van der Ley P., Bekkers A., Van Meersbergen J., Tommassen J. A comparative study on the phoE genes of three enterobacterial species. Implications for structure-function relationships in a pore-forming protein of the outer membrane. Eur J Biochem. 1987 Apr 15;164(2):469–475. doi: 10.1111/j.1432-1033.1987.tb11080.x. [DOI] [PubMed] [Google Scholar]
  12. van der Ley P., Struyvé M., Tommassen J. Topology of outer membrane pore protein PhoE of Escherichia coli. Identification of cell surface-exposed amino acids with the aid of monoclonal antibodies. J Biol Chem. 1986 Sep 15;261(26):12222–12225. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES