Skip to main content
PMC home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1972 Mar;66(3):445–460.

Platelets in Early Hyperacute Allograft Rejection in Kidneys and Their Modification by Sulfinpyrazone (Anturan) Therapy

An Experimental Study

Hari M Sharma, S Moore, H W Merrick, M R Smith
PMCID: PMC2032721  PMID: 4551295

Abstract

Experiments were carried out on mongrel dogs to study the early ultrastructural changes in hyperacute rejection of kidney. Renal allografts were performed on dogs presensitized by three to five skin grafts. The animals were grouped in pairs, control and treated with sulfinpyrazone, a platelet inhibitor. The electron microscopic studies showed that the earliest change in the kidney was aggregation of platelets mainly in the peritubular capillaries and a few glomeruli. Later, platelets showed degranulation with vascular damage leading to rejection of kidney. Treated dogs showed less tendency for platelets to aggregate, and graft survival was prolonged. The role or platelets in the hyperacute rejection is discussed.

Full text

PDF
445

Images in this article

460Fig 6
on p.460
455Fig 1
on p.455
456Fig 2
on p.456
457Fig 3
on p.457
458Fig 4
on p.458
459Fig 5
on p.459

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALTMAN B. TISSUE TRANSPLANTATION: CIRCULATING ANTIBODY IN THE HOMOTRANSPLANTATION OF KIDNEY AND SKIN. Ann R Coll Surg Engl. 1963 Aug;33:79–104. [PMC free article] [PubMed] [Google Scholar]
  2. BETTEX-GALLAND M., LUESCHER E. F. UNTERSUCHUNGEN UEBER DIE AUSLOESUNG DER VISKOESEN METAMORPHOSE DER MENSCHLICHEN BLUTPLAETTCHEN DURCH IMMUNKOMPLEXE. Pathol Microbiol (Basel) 1964;27:533–541. [PubMed] [Google Scholar]
  3. BUCKINGHAM S., MAYNERT E. W. THE RELEASE OF 5-HYDROXYTRYPTAMINE, POTASSIUM AND AMINO ACIDS FROM PLATELETS. J Pharmacol Exp Ther. 1964 Mar;143:332–339. [PubMed] [Google Scholar]
  4. Busch G. J., Braun W. E., Carpenter C. B., Corson J. M., Galvanek E. R., Reynolds E. S., Merrill J. P., Dammin G. J. Intravascular coagulation (IVC) in human renal allograft rejection. Transplant Proc. 1969 Mar;1(1):267–270. [PubMed] [Google Scholar]
  5. Busch G. J., Braun W. E., Carpenter C. B., Corson J. M., Galvanek E. R., Reynolds E. S., Merrill J. P., Dammin G. J. Intravascular coagulation (IVC) in human renal allograft rejection. Transplant Proc. 1969 Mar;1(1):267–270. [PubMed] [Google Scholar]
  6. Busch G. J., Reynolds E. S., Galvanek E. G., Braun W. E., Dammin G. J. Human renal allografts. The role of vascular injury in early graft failure. Medicine (Baltimore) 1971 Jan;50(1):29–83. [PubMed] [Google Scholar]
  7. Dubernard J. M., Carpenter C. B., Busch G. J., Diethelm A. G., Murray J. E. Rejection of canine renal allografts by passive transfer of sensitized serum. Surgery. 1968 Oct;64(4):752–760. [PubMed] [Google Scholar]
  8. HUMPHREY J. H., JAQUES R. The release of histamine and 5-hydroxytryptamine (serotonin) from platelets by antigen-antibody reactions (in vitro). J Physiol. 1955 Apr 28;128(1):9–27. doi: 10.1113/jphysiol.1955.sp005289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hinterberger H., Anthony M., Vagholkar M. K. Platelet 5-hydroxytryptamine and adenine nucleotides, serum arginylesterase and plasma 11-hydroxycorticosteroids in migraine. Clin Sci. 1968 Apr;34(2):271–276. [PubMed] [Google Scholar]
  11. Holl-Allen R. T., Scharli A., Rippin A., Busch G. J., Simonian S. J., Wilson R. E. Cytotoxic antibody after antigen pretreatment: enhancement of renal allografts. Surg Forum. 1969;20:276–278. [PubMed] [Google Scholar]
  12. ISHIZAKA T., ISHIZAKA K. Biological activities of aggregated gamma-globulin. V. Agglutination of erythrocytes and platelets. J Immunol. 1962 Nov;89:709–716. [PubMed] [Google Scholar]
  13. Jorgensen L., Rowsell H. C., Hovig T., Glynn M. F., Mustard J. F. Adenosine diphosphate-induced platelet aggregation and myocardial infarction in swine. Lab Invest. 1967 Dec;17(6):616–644. [PubMed] [Google Scholar]
  14. Kahan B. D., Reisfeld R. A., Pellegrino M. A., Curtoni E. S., Mattiuz P. L., Ceppellini R. Water-soluble human transplantation antigen. Proc Natl Acad Sci U S A. 1968 Nov;61(3):897–904. doi: 10.1073/pnas.61.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kincaid-Smith P., McKenzie I. F., Morris P. J., Marshall V. C. Biopsy features of early acute rejection in cadaveric renal grafts. Transplant Proc. 1969 Mar;1(1):287–289. [PubMed] [Google Scholar]
  16. Kissmeyer-Nielsen F., Olsen S., Petersen V. P., Fjeldborg O. Hyperacute rejection of kidney allografts, associated with pre-existing humoral antibodies against donor cells. Lancet. 1966 Sep 24;2(7465):662–665. doi: 10.1016/s0140-6736(66)92829-7. [DOI] [PubMed] [Google Scholar]
  17. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Legrand Y., Robert B., Szigeti M., Pignaud G., Caen J., Robert L. Etudes sur une protéase élastinolytique des plaquettes anguines humaines. Atherosclerosis. 1970 Nov-Dec;12(3):451–465. doi: 10.1016/0021-9150(70)90049-3. [DOI] [PubMed] [Google Scholar]
  19. Lowenhaupt R., Nathan P. Platelet accumulation observed by electron microscopy in the early phase of renal allotransplant rejection. Nature. 1968 Nov 23;220(5169):822–825. doi: 10.1038/220822b0. [DOI] [PubMed] [Google Scholar]
  20. Mann D. L., Rogentine G. N., Fahey J. L., Nathenson S. Human lymphocyte membrane (HL-A) alloantigens: isolation, purification and properties. J Immunol. 1969 Aug;103(2):282–292. [PubMed] [Google Scholar]
  21. Mills D. C., Robb I. A., Roberts G. C. The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. J Physiol. 1968 Apr;195(3):715–729. doi: 10.1113/jphysiol.1968.sp008484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Moore S., Lough J. Lipid accumulation in renal arterioles due to platelet aggregate embolism. Am J Pathol. 1970 Feb;58(2):283–293. [PMC free article] [PubMed] [Google Scholar]
  23. Movat H. Z., Mustard J. F., Taichman N. S., Uriuhara T. Platelet aggregation and release of ADP, serotonin and histamine associated with phagocytosis of antigen-antibody complexes. Proc Soc Exp Biol Med. 1965 Oct;120(1):232–237. doi: 10.3181/00379727-120-30496. [DOI] [PubMed] [Google Scholar]
  24. Movat H. Z., Mustard J. F., Taichman N. S., Uriuhara T. Platelet aggregation and release of ADP, serotonin and histamine associated with phagocytosis of antigen-antibody complexes. Proc Soc Exp Biol Med. 1965 Oct;120(1):232–237. doi: 10.3181/00379727-120-30496. [DOI] [PubMed] [Google Scholar]
  25. PACHTER M. R., JOHNSON S. A., NEBLETT T. R., TRUANT J. P. Bleeding platelets, and macroglobulinemia. Am J Clin Pathol. 1959 Jun;31(6):467–482. doi: 10.1093/ajcp/31.6.467. [DOI] [PubMed] [Google Scholar]
  26. Packham M. A., Warrior E. S., Glynn M. F., Senyi A. S., Mustard J. F. Alteration of the response of platelets to surface stimuli by pyrazole compounds. J Exp Med. 1967 Jul 1;126(1):171–188. doi: 10.1084/jem.126.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Patel R., Terasaki P. I. Significance of the positive crossmatch test in kidney transplantation. N Engl J Med. 1969 Apr 3;280(14):735–739. doi: 10.1056/NEJM196904032801401. [DOI] [PubMed] [Google Scholar]
  28. RAPAPORT F. T., DAUSSET J., CONVERSE J. M., LAWRENCE H. S. BIOLOGICAL AND ULTRASTRUCTURAL STUDIES OF LEUCOCYTE FRACTIONS AS TRANSPLANTATION ANTIGENS IN MAN. Transplantation. 1965 Jul;3:490–500. doi: 10.1097/00007890-196507000-00004. [DOI] [PubMed] [Google Scholar]
  29. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SMYTHE H. A., OGRYZLO M. A., MURPHY E. A., MUSTARD J. F. THE EFFECT OF SULFINPYRAZONE (ANTURAN) ON PLATELET ECONOMY AND BLOOD COAGULATION IN MAN. Can Med Assoc J. 1965 Apr 10;92:818–821. [PMC free article] [PubMed] [Google Scholar]
  31. Simpson K. M., Bunch D. L., Amemiya H., Boehmig H. J., Wilson C. B., Dixon F. J., Coburg A. J., Hathaway W. E., Giles G. R., Starzl T. E. Humoral antibodies and coagulation mechanisms in the accelerated or hyperacute rejection of renal homografts in sensitized canine recipients. Surgery. 1970 Jul;68(1):77–85. [PMC free article] [PubMed] [Google Scholar]
  32. Starzl T. E., Lerner R. A., Dixon F. J., Groth C. G., Brettschneider L., Terasaki P. I. Shwartzman reaction after human renal homotransplantation. N Engl J Med. 1968 Mar 21;278(12):642–648. doi: 10.1056/NEJM196803212781202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Williams G. M., Hume D. M., Hudson R. P., Jr, Morris P. J., Kano K., Milgrom F. "Hyperacute" renal-homograft rejection in man. N Engl J Med. 1968 Sep 19;279(12):611–618. doi: 10.1056/NEJM196809192791201. [DOI] [PubMed] [Google Scholar]
  34. Wilson R. E., Rippin A., Dagher R. K., Kinneart P., Busch G. J. Prolonged cancine renal allograft survival after pretreatment with solubilized antigen. Transplantation. 1969 May;7(5):360–371. doi: 10.1097/00007890-196905000-00005. [DOI] [PubMed] [Google Scholar]
  35. Yamada T., Kay J. H. Kidney homotransplantation with special reference to cytotoxic antibody response. Surgery. 1968 Apr;63(4):637–645. [PubMed] [Google Scholar]
  36. ZIEVE P. D., GAMBLE J. L., Jr, JACKSON D. P. EFFECTS OF THROMBIN ON THE POTASSIUM AND ATP CONTENT OF PLATELETS. J Clin Invest. 1964 Nov;43:2063–2069. doi: 10.1172/JCI105080. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES