Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1972 Mar;66(3):497–512.

Effect of Staphylococcal α-Toxin on the Fine Structure of Pancreatic Acinar Cells of Dogs

Polly Schoning, N V Anderson, Jane A Westfall
PMCID: PMC2032734  PMID: 4334216

Abstract

Staphylococcal α-toxin induced pancreatitis in dogs was studied by electron microscopy. Pancreatic acinar cell injury was indicated by increased vesiculation of endoplasmic reticulum, loss of intracisternal granules, changes in mitochondria, membrane discontinuities and an increased number of dense complex bodies, lipid droplets and free ribosomes.

Full text

PDF
497

Images in this article

507Fig 3
on p.507
508Fig 4
on p.508
509Fig 5
on p.509
510Fig 6
on p.510
511Fig 7
on p.511
511Fig 8
on p.511
505Fig 1
on p.505
506Fig 2
on p.506

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alousi M. A., Stenger R. J., Morgan W. S. The fine structure of pancreatic acinar and hepatic parenchymal cells after neutral red dye injection. Exp Mol Pathol. 1968 Aug;9(1):97–109. doi: 10.1016/0014-4800(68)90054-3. [DOI] [PubMed] [Google Scholar]
  2. Boquist L. Morphologic effects of ethionine on the pancreas of the Chinese hamster. A light and electron microscopic study of degenerative changes. Acta Pathol Microbiol Scand. 1969;76(1):91–105. doi: 10.1111/j.1699-0463.1969.tb03237.x. [DOI] [PubMed] [Google Scholar]
  3. CELLE G., CUNEO P. ASPETTI ULTRAMICROSCOPICI DEL PANCREAS UMANO NORMALE (PARTE ESOCRINA) Arch Maragliano Patol Clin. 1964 Dec;20:489–504. [PubMed] [Google Scholar]
  4. Fedou R., Ribet A., Izard J. Corpuscles containing crystalline formations in the exocrine pancreas of the dog. Exp Cell Res. 1966 May;42(2):389–391. doi: 10.1016/0014-4827(66)90306-5. [DOI] [PubMed] [Google Scholar]
  5. Fitzgerald P. J., Herman L., Carol B., Roque A., Marsh W. H., Rosenstock L., Richards C., Perl D. Pancreatic acinar cell regeneration. Am J Pathol. 1968 May;52(5):983–1011. [PMC free article] [PubMed] [Google Scholar]
  6. HERMAN L., FITZGERALD P. J. The degenerative changes in pancreatic acinar cells caused by DL-ethionine. J Cell Biol. 1962 Feb;12:277–296. doi: 10.1083/jcb.12.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HRUBAN Z., SWIFT H., DUNN F. W., LEWIS D. E. EFFECT OF BETA-3-FURYLALANINE ON THE ULTRASTRUCTURE OF THE HEPATOCYTES AND PANCREATIC ACINAR CELLS. Lab Invest. 1965 Jan;14:70–80. [PubMed] [Google Scholar]
  8. HRUBAN Z., SWIFT H., WISSLER R. W. Effect of beta-3-thienylalanine on the formation of zymogen granules of exocrine pancreas. J Ultrastruct Res. 1962 Oct;7:359–372. doi: 10.1016/s0022-5320(62)90031-x. [DOI] [PubMed] [Google Scholar]
  9. Hruban Z., Swift H., Slesers A. Effect of triparanol and diethanolamine on the fine structure of hepatocytes and pancreatic acinar cells. Lab Invest. 1965 Sep;14(9):1652–1672. [PubMed] [Google Scholar]
  10. ICHIKAWA A. FINE STRUCTURAL CHANGES IN RESPONSE TO HORMONAL STIMULATION OF THE PERFUSED CANINE PANCREAS. J Cell Biol. 1965 Mar;24:369–385. doi: 10.1083/jcb.24.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imai M. Ultrastructural changes of pancreatic acinar cells following antibiotics administration. Experiments of guinea pigs with chloramphenicol and tetracycline. Nagoya J Med Sci. 1966 Jun;28(3):247–260. [PubMed] [Google Scholar]
  12. Jamieson J. D., Palade G. E. Intracellular transport of secretory proteins in the pancreatic exocrine cell. IV. Metabolic requirements. J Cell Biol. 1968 Dec;39(3):589–603. doi: 10.1083/jcb.39.3.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kern H. F., Kern D. Elektronenmikroskopische Untersuchungen über die Wirkung von Kobaltchlorid auf das exokrine Pankreasgewebe des Meerschweinchens. Virchows Arch B Cell Pathol. 1969;4(1):54–70. [PubMed] [Google Scholar]
  14. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Longnecker D. S., Shinozuka H., Farber E. Molecular pathology of in-vivo inhibition of protein synthesis. Electron microscopy of rat pancreatic acinar cells in puromycin-induced necrosis. Am J Pathol. 1968 Apr;52(4):891–915. [PMC free article] [PubMed] [Google Scholar]
  16. Marsh W. H., Goldsmith S., Crocco J., Fitzgerald P. J. Pancreatic acinar cell regeneration. II. Enzymatic, nucleic acid, and protein changes. Am J Pathol. 1968 May;52(5):1013–1037. [PMC free article] [PubMed] [Google Scholar]
  17. PALADE G. E. Intracisternal granules in the exocrine cells of the pancreas. J Biophys Biochem Cytol. 1956 Jul 25;2(4):417–422. doi: 10.1083/jcb.2.4.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. THAL A., MOLESTINA J. E. Studies on pancreatitis. III. Fulminating hemorrhagic pancreatic necrosis produced by means of staphylococcal toxin. AMA Arch Pathol. 1955 Aug;60(2):212–220. [PubMed] [Google Scholar]
  20. Tardini A., Anversa P., Bordi C., Bertaccini G., Impicciatore M. Ultrastructural and biochemical changes after marked caerulein stimulation of the exocrine pancreas in the dog. Am J Pathol. 1971 Jan;62(1):35–56. [PMC free article] [PubMed] [Google Scholar]
  21. Volk B. W., Wellmann K. F., Lewitan A. The effect of irradiation on the fine structure and enzymes of the dog pancreas. I. Short-term studies. Am J Pathol. 1966 May;48(5):721–753. [PMC free article] [PubMed] [Google Scholar]
  22. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watari N., Baba N. Several findings on the fine structural alterations of the exocrine pancreas after the administration of some chemicals. J Electron Microsc (Tokyo) 1968;17(4):327–341. [PubMed] [Google Scholar]
  24. Weinstock A. Cytotoxic effects of puromycin on the Golgi apparatus of pancreatic acinar cells, hepatocytes and ameloblasts. J Histochem Cytochem. 1970 Dec;18(12):875–886. doi: 10.1177/18.12.875. [DOI] [PubMed] [Google Scholar]
  25. ZELANDER T., EKHOLM R., EDLUND Y. THE ULTRASTRUCTURE OF THE RAT EXOCRINE PANCREAS AFTER EXPERIMENTALLY OCCLUDED OUTFLOW. J Ultrastruct Res. 1964 Feb;10:89–102. doi: 10.1016/s0022-5320(64)90023-1. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES