Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1972 Dec;69(3):389–406.

Differentiation of Blood Group H in a Human Transformed Cell Line

William J Kuhns, Charlotte Pann, Sharon Bramson
PMCID: PMC2032802  PMID: 4564036

Abstract

HeLa cells were separated into two populations by agglutination with anti-H ulex reagent which was defined according to its reactivity against L-fucose, the immunodeterminant sugar for blood group H. Chromosome studies of individual cells from each population indicated that they were karyotypically similar and that they possessed a single modal chromosome number of 69. Studies of tissue culture supernates and of cellular fractions and their supernates showed that HeLa cell group H was membrane associated and not soluble in nature. Cloning studies of single H- cells separated from H- cells agglutinated by anti-H demonstrated progeny comprised of mixed H-/H- cells, a finding which was supported by results of population studies on typed cells grown in monolayer culture. It is believed that the H- population represents germinal cells that are capable of dividing to form mixed H-/H- cellular progeny, the former representing a more fully differentiated cell form, which eventually exfoliates and becomes nonfunctional.

Full text

PDF
389

Images in this article

405Figs 3 and 4
on p.405
406Fig 5
on p.406
406Fig 6
on p.406
403Fig 1
on p.403
403Fig 2
on p.403

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYD W. C., SHAPLEIGH E. Diagnosis by subgroups of blood groups A and AB by use of plant agglutinins (lectins). J Lab Clin Med. 1954 Aug;44(2):235–237. [PubMed] [Google Scholar]
  2. CHESSIN L. N., BRAMSON S., KUHNS W. J., HIRSCHHORN K. STUDIES ON THE A, B, O(H) BLOOD GROUPS ON HUMAN CELLS IN CULTURE. Blood. 1965 Jun;25:944–953. [PubMed] [Google Scholar]
  3. EDWARDS R. G., FERGUSON L. C., COOMBS R. R. BLOOD GROUP ATINGENS ON HUMAN SPERMATOZOA. J Reprod Fertil. 1964 Apr;7:153–161. doi: 10.1530/jrf.0.0070153. [DOI] [PubMed] [Google Scholar]
  4. FENTON J. W., 2nd, DUGGLEBY C. R., OTTEN C., STONE W. H. ISOLATION AND FLUORESCENT LABELING OF ULEX EUROPAEUS ANTI-H LECTIN. Vox Sang. 1965 Mar-Apr;10:208–211. doi: 10.1111/j.1423-0410.1965.tb04339.x. [DOI] [PubMed] [Google Scholar]
  5. Franks D., Dawson A. Variation in the expression of blood group antigen A in clonal cultures of rabbit cells. Exp Cell Res. 1966 Jun;42(3):543–561. doi: 10.1016/0014-4827(66)90268-0. [DOI] [PubMed] [Google Scholar]
  6. HOLBOROW E. J., BROWN P. C., GLYNN L. E., HAWES M. D., GRESHAM G. A., O'BRIEN T. F., COOMBS R. R. The distribution of the blood group. A antigen in human tissues. Br J Exp Pathol. 1960 Aug;41:430–437. [PMC free article] [PubMed] [Google Scholar]
  7. KELUS A., GURNER B. W., COOMBS R. R. Blood group antigens on HeLa cells shown by mixed agglutination. Immunology. 1959 Jul;2:262–267. [PMC free article] [PubMed] [Google Scholar]
  8. Kovarik S., Davidsohn I., Stejskal R. ABO antigens in cancer. Detection with the mixed cell agglutination reaction. Arch Pathol. 1968 Jul;86(1):12–21. [PubMed] [Google Scholar]
  9. Kuhns W. J., Faur Y. Cellular variants for blood groups within an established cell line. Proc Soc Exp Biol Med. 1970 Feb;133(2):601–606. doi: 10.3181/00379727-133-34526. [DOI] [PubMed] [Google Scholar]
  10. LEVINTOW L., DARNELL J. E., Jr A simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of Type 1 poliovirus. J Biol Chem. 1960 Jan;235:70–73. [PubMed] [Google Scholar]
  11. MORGAN W. T. J., WATKINS W. M. The inhibition of the haemagglutinins in plant seeds by human blood group substances and simple sugars. Br J Exp Pathol. 1953 Feb;34(1):94–103. [PMC free article] [PubMed] [Google Scholar]
  12. Moscona A. A. Embryonic and neoplastic cell surfaces: availability of receptors for concanavalin A and wheat germ agglutinin. Science. 1971 Mar 5;171(3974):905–907. doi: 10.1126/science.171.3974.905. [DOI] [PubMed] [Google Scholar]
  13. TJIO J. H., PUCK T. T. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med. 1958 Aug 1;108(2):259–268. doi: 10.1084/jem.108.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES