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. 1994 Jul;70(1):18–20. doi: 10.1038/bjc.1994.243

Deletion analysis of chromosome 8p in sporadic colorectal adenomas.

C Cunningham 1, M G Dunlop 1, C C Bird 1, A H Wyllie 1
PMCID: PMC2033333  PMID: 8018534

Abstract

In order to assess the stage of colorectal tumorigenesis at which chromosome 8p loss of heterozygosity (LOH) occurs, 56 sporadic adenomas were examined for LOH at four polymorphic loci which show frequent LOH in carcinomas. LOH was found in only 5 out of 51 (9.8%) informative adenomas, whereas studies with the same markers in 85 informative carcinomas showed a LOH of 45%. The adenomas showing LOH were all in the 'high-risk' clinicopathological category, being 10 mm or more in diameter and showing tubulovillous architecture. It is concluded that the chromosome 8p locus is involved preferentially in the development of carcinomas rather than adenomas.

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Selected References

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  1. Aaltonen L. A., Peltomäki P., Leach F. S., Sistonen P., Pylkkänen L., Mecklin J. P., Järvinen H., Powell S. M., Jen J., Hamilton S. R. Clues to the pathogenesis of familial colorectal cancer. Science. 1993 May 7;260(5109):812–816. doi: 10.1126/science.8484121. [DOI] [PubMed] [Google Scholar]
  2. Baker S. J., Preisinger A. C., Jessup J. M., Paraskeva C., Markowitz S., Willson J. K., Hamilton S., Vogelstein B. p53 gene mutations occur in combination with 17p allelic deletions as late events in colorectal tumorigenesis. Cancer Res. 1990 Dec 1;50(23):7717–7722. [PubMed] [Google Scholar]
  3. Bergerheim U. S., Kunimi K., Collins V. P., Ekman P. Deletion mapping of chromosomes 8, 10, and 16 in human prostatic carcinoma. Genes Chromosomes Cancer. 1991 May;3(3):215–220. doi: 10.1002/gcc.2870030308. [DOI] [PubMed] [Google Scholar]
  4. Bruin T., Reymer P. W., Groenemeyer B. E., Talmud P. J., Kastelein J. J. HindIII-polymorphism in the LPL-gene detected by PCR. Nucleic Acids Res. 1991 Nov 25;19(22):6346–6346. doi: 10.1093/nar/19.22.6346-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carder P., Wyllie A. H., Purdie C. A., Morris R. G., White S., Piris J., Bird C. C. Stabilised p53 facilitates aneuploid clonal divergence in colorectal cancer. Oncogene. 1993 May;8(5):1397–1401. [PubMed] [Google Scholar]
  6. Cunningham C., Dunlop M. G., Wyllie A. H., Bird C. C. Deletion mapping in colorectal cancer of a putative tumour suppressor gene in 8p22-p21.3. Oncogene. 1993 May;8(5):1391–1396. [PubMed] [Google Scholar]
  7. Emi M., Fujiwara Y., Nakajima T., Tsuchiya E., Tsuda H., Hirohashi S., Maeda Y., Tsuruta K., Miyaki M., Nakamura Y. Frequent loss of heterozygosity for loci on chromosome 8p in hepatocellular carcinoma, colorectal cancer, and lung cancer. Cancer Res. 1992 Oct 1;52(19):5368–5372. [PubMed] [Google Scholar]
  8. Emi M., Fujiwara Y., Ohata H., Tsuda H., Hirohashi S., Koike M., Miyaki M., Monden M., Nakamura Y. Allelic loss at chromosome band 8p21.3-p22 is associated with progression of hepatocellular carcinoma. Genes Chromosomes Cancer. 1993 Jul;7(3):152–157. doi: 10.1002/gcc.2870070307. [DOI] [PubMed] [Google Scholar]
  9. Fearon E. R., Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990 Jun 1;61(5):759–767. doi: 10.1016/0092-8674(90)90186-i. [DOI] [PubMed] [Google Scholar]
  10. Fishel R., Lescoe M. K., Rao M. R., Copeland N. G., Jenkins N. A., Garber J., Kane M., Kolodner R. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer. Cell. 1993 Dec 3;75(5):1027–1038. doi: 10.1016/0092-8674(93)90546-3. [DOI] [PubMed] [Google Scholar]
  11. Fujiwara Y., Emi M., Ohata H., Kato Y., Nakajima T., Mori T., Nakamura Y. Evidence for the presence of two tumor suppressor genes on chromosome 8p for colorectal carcinoma. Cancer Res. 1993 Mar 1;53(5):1172–1174. [PubMed] [Google Scholar]
  12. Ichii S., Takeda S., Horii A., Nakatsuru S., Miyoshi Y., Emi M., Fujiwara Y., Koyama K., Furuyama J., Utsunomiya J. Detailed analysis of genetic alterations in colorectal tumors from patients with and without familial adenomatous polyposis (FAP). Oncogene. 1993 Sep;8(9):2399–2405. [PubMed] [Google Scholar]
  13. Ionov Y., Peinado M. A., Malkhosyan S., Shibata D., Perucho M. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. Nature. 1993 Jun 10;363(6429):558–561. doi: 10.1038/363558a0. [DOI] [PubMed] [Google Scholar]
  14. Kikuchi-Yanoshita R., Konishi M., Ito S., Seki M., Tanaka K., Maeda Y., Iino H., Fukayama M., Koike M., Mori T. Genetic changes of both p53 alleles associated with the conversion from colorectal adenoma to early carcinoma in familial adenomatous polyposis and non-familial adenomatous polyposis patients. Cancer Res. 1992 Jul 15;52(14):3965–3971. [PubMed] [Google Scholar]
  15. Knowles M. A., Shaw M. E., Proctor A. J. Deletion mapping of chromosome 8 in cancers of the urinary bladder using restriction fragment length polymorphisms and microsatellite polymorphisms. Oncogene. 1993 May;8(5):1357–1364. [PubMed] [Google Scholar]
  16. Leach F. S., Nicolaides N. C., Papadopoulos N., Liu B., Jen J., Parsons R., Peltomäki P., Sistonen P., Aaltonen L. A., Nyström-Lahti M. Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer. Cell. 1993 Dec 17;75(6):1215–1225. doi: 10.1016/0092-8674(93)90330-s. [DOI] [PubMed] [Google Scholar]
  17. Livingstone L. R., White A., Sprouse J., Livanos E., Jacks T., Tlsty T. D. Altered cell cycle arrest and gene amplification potential accompany loss of wild-type p53. Cell. 1992 Sep 18;70(6):923–935. doi: 10.1016/0092-8674(92)90243-6. [DOI] [PubMed] [Google Scholar]
  18. Polymeropoulos M. H., Rath D. S., Xiao H., Merril C. R. Dinucleotide repeat polymorphism at the human ankyrin gene (ANK1). Nucleic Acids Res. 1991 Feb 25;19(4):969–969. doi: 10.1093/nar/19.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Powell S. M., Zilz N., Beazer-Barclay Y., Bryan T. M., Hamilton S. R., Thibodeau S. N., Vogelstein B., Kinzler K. W. APC mutations occur early during colorectal tumorigenesis. Nature. 1992 Sep 17;359(6392):235–237. doi: 10.1038/359235a0. [DOI] [PubMed] [Google Scholar]
  20. Thibodeau S. N., Bren G., Schaid D. Microsatellite instability in cancer of the proximal colon. Science. 1993 May 7;260(5109):816–819. doi: 10.1126/science.8484122. [DOI] [PubMed] [Google Scholar]
  21. Tomfohrde J., Wood S., Schertzer M., Wagner M. J., Wells D. E., Parrish J., Sadler L. A., Blanton S. H., Daiger S. P., Wang Z. Human chromosome 8 linkage map based on short tandem repeat polymorphisms: effect of genotyping errors. Genomics. 1992 Sep;14(1):144–152. doi: 10.1016/s0888-7543(05)80297-6. [DOI] [PubMed] [Google Scholar]
  22. Young J., Leggett B., Gustafson C., Ward M., Searle J., Thomas L., Buttenshaw R., Chenevix-Trench G. Genomic instability occurs in colorectal carcinomas but not in adenomas. Hum Mutat. 1993;2(5):351–354. doi: 10.1002/humu.1380020505. [DOI] [PubMed] [Google Scholar]

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