Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1995 Feb;71(2):344–349. doi: 10.1038/bjc.1995.69

Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.

I Kinoshita 1, H Dosaka-Akita 1, M Shindoh 1, M Fujino 1, K Akie 1, M Kato 1, K Fujinaga 1, Y Kawakami 1
PMCID: PMC2033613  PMID: 7841051

Abstract

To provide an accurate evaluation of the association of human papillomavirus (HPV) with lung cancer, 36 cases of lung cancer were analysed for HPV DNAs by polymerase chain reaction (PCR) with dot-blot and Southern blot analyses, and for the transcripts from the E6-E7 transforming region by in situ hybridisation (ISH). HPV-18 DNA was detected in three (8%) of 36 specimens; histologically, in one (10%) of 10 squamous cell carcinomas and two (9%) of 22 adenocarcinomas. Neither HPV-16 nor -33 DNA was detected in any cases examined. Expression of E6-E7 mRNA was confirmed in the cases which contained, HPV-18 DNA. HPV-18 may play an important role in the development and progression of cancer in some cases of both squamous cell carcinoma and adenocarcinoma of the lung.

Full text

PDF
344

Images in this article

346Figure 1
on p.346
346Figure 2
on p.346
346Figure 3
on p.346
347Figure 4
on p.347
347Figure 5
on p.347

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anwar K., Nakakuki K., Naiki H., Inuzuka M. ras gene mutations and HPV infection are common in human laryngeal carcinoma. Int J Cancer. 1993 Jan 2;53(1):22–28. doi: 10.1002/ijc.2910530106. [DOI] [PubMed] [Google Scholar]
  2. Band V., Zajchowski D., Kulesa V., Sager R. Human papilloma virus DNAs immortalize normal human mammary epithelial cells and reduce their growth factor requirements. Proc Natl Acad Sci U S A. 1990 Jan;87(1):463–467. doi: 10.1073/pnas.87.1.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Béjui-Thivolet F., Liagre N., Chignol M. C., Chardonnet Y., Patricot L. M. Detection of human papillomavirus DNA in squamous bronchial metaplasia and squamous cell carcinomas of the lung by in situ hybridization using biotinylated probes in paraffin-embedded specimens. Hum Pathol. 1990 Jan;21(1):111–116. doi: 10.1016/0046-8177(90)90082-g. [DOI] [PubMed] [Google Scholar]
  4. Chiba I., Takahashi T., Nau M. M., D'Amico D., Curiel D. T., Mitsudomi T., Buchhagen D. L., Carbone D., Piantadosi S., Koga H. Mutations in the p53 gene are frequent in primary, resected non-small cell lung cancer. Lung Cancer Study Group. Oncogene. 1990 Oct;5(10):1603–1610. [PubMed] [Google Scholar]
  5. Harbour J. W., Lai S. L., Whang-Peng J., Gazdar A. F., Minna J. D., Kaye F. J. Abnormalities in structure and expression of the human retinoblastoma gene in SCLC. Science. 1988 Jul 15;241(4863):353–357. doi: 10.1126/science.2838909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hawley-Nelson P., Vousden K. H., Hubbert N. L., Lowy D. R., Schiller J. T. HPV16 E6 and E7 proteins cooperate to immortalize human foreskin keratinocytes. EMBO J. 1989 Dec 1;8(12):3905–3910. doi: 10.1002/j.1460-2075.1989.tb08570.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Higgins G. D., Uzelin D. M., Phillips G. E., Pieterse A. S., Burrell C. J. Differing characteristics of human papillomavirus RNA-positive and RNA-negative anal carcinomas. Cancer. 1991 Aug 1;68(3):561–567. doi: 10.1002/1097-0142(19910801)68:3<561::aid-cncr2820680320>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]
  8. Münger K., Werness B. A., Dyson N., Phelps W. C., Harlow E., Howley P. M. Complex formation of human papillomavirus E7 proteins with the retinoblastoma tumor suppressor gene product. EMBO J. 1989 Dec 20;8(13):4099–4105. doi: 10.1002/j.1460-2075.1989.tb08594.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ostrow R. S., Manias D. A., Fong W. J., Zachow K. R., Faras A. J. A survey of human cancers for human papillomavirus DNA by filter hybridization. Cancer. 1987 Feb 1;59(3):429–434. doi: 10.1002/1097-0142(19870201)59:3<429::aid-cncr2820590313>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  10. Pietenpol J. A., Stein R. W., Moran E., Yaciuk P., Schlegel R., Lyons R. M., Pittelkow M. R., Münger K., Howley P. M., Moses H. L. TGF-beta 1 inhibition of c-myc transcription and growth in keratinocytes is abrogated by viral transforming proteins with pRB binding domains. Cell. 1990 Jun 1;61(5):777–785. doi: 10.1016/0092-8674(90)90188-k. [DOI] [PubMed] [Google Scholar]
  11. Popper H. H., Wirnsberger G., Jüttner-Smolle F. M., Pongratz M. G., Sommersgutter M. The predictive value of human papilloma virus (HPV) typing in the prognosis of bronchial squamous cell papillomas. Histopathology. 1992 Oct;21(4):323–330. doi: 10.1111/j.1365-2559.1992.tb00402.x. [DOI] [PubMed] [Google Scholar]
  12. Reissmann P. T., Koga H., Takahashi R., Figlin R. A., Holmes E. C., Piantadosi S., Cordon-Cardo C., Slamon D. J. Inactivation of the retinoblastoma susceptibility gene in non-small-cell lung cancer. The Lung Cancer Study Group. Oncogene. 1993 Jul;8(7):1913–1919. [PubMed] [Google Scholar]
  13. Scheffner M., Werness B. A., Huibregtse J. M., Levine A. J., Howley P. M. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell. 1990 Dec 21;63(6):1129–1136. doi: 10.1016/0092-8674(90)90409-8. [DOI] [PubMed] [Google Scholar]
  14. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature. 1985 Mar 7;314(6006):111–114. doi: 10.1038/314111a0. [DOI] [PubMed] [Google Scholar]
  15. Shamanin V., Delius H., de Villiers E. M. Development of a broad spectrum PCR assay for papillomaviruses and its application in screening lung cancer biopsies. J Gen Virol. 1994 May;75(Pt 5):1149–1156. doi: 10.1099/0022-1317-75-5-1149. [DOI] [PubMed] [Google Scholar]
  16. Shimizu S., Sabsay B., Veis A., Ostrow J. D., Rege R. V., Dawes L. G. Isolation of an acidic protein from cholesterol gallstones, which inhibits the precipitation of calcium carbonate in vitro. J Clin Invest. 1989 Dec;84(6):1990–1996. doi: 10.1172/JCI114389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shindoh M., Sawada Y., Kohgo T., Amemiya A., Fujinaga K. Detection of human papillomavirus DNA sequences in tongue squamous-cell carcinoma utilizing the polymerase chain reaction method. Int J Cancer. 1992 Jan 21;50(2):167–171. doi: 10.1002/ijc.2910500202. [DOI] [PubMed] [Google Scholar]
  18. Stoler M. H., Mills S. E., Gersell D. J., Walker A. N. Small-cell neuroendocrine carcinoma of the cervix. A human papillomavirus type 18-associated cancer. Am J Surg Pathol. 1991 Jan;15(1):28–32. doi: 10.1097/00000478-199101000-00003. [DOI] [PubMed] [Google Scholar]
  19. Stoler M. H., Rhodes C. R., Whitbeck A., Wolinsky S. M., Chow L. T., Broker T. R. Human papillomavirus type 16 and 18 gene expression in cervical neoplasias. Hum Pathol. 1992 Feb;23(2):117–128. doi: 10.1016/0046-8177(92)90232-r. [DOI] [PubMed] [Google Scholar]
  20. Storey A., Pim D., Murray A., Osborn K., Banks L., Crawford L. Comparison of the in vitro transforming activities of human papillomavirus types. EMBO J. 1988 Jun;7(6):1815–1820. doi: 10.1002/j.1460-2075.1988.tb03013.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stremlau A., Gissmann L., Ikenberg H., Stark M., Bannasch P., zur Hausen H. Human papillomavirus type 16 related DNA in an anaplastic carcinoma of the lung. Cancer. 1985 Apr 15;55(8):1737–1740. doi: 10.1002/1097-0142(19850415)55:8<1737::aid-cncr2820550820>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  22. Syrjänen K., Syrjänen S., Kellokoski J., Kärjä J., Mäntyjärvi R. Human papillomavirus (HPV) type 6 and 16 DNA sequences in bronchial squamous cell carcinomas demonstrated by in situ DNA hybridization. Lung. 1989;167(1):33–42. doi: 10.1007/BF02714928. [DOI] [PubMed] [Google Scholar]
  23. Szabó I., Sepp R., Nakamoto K., Maeda M., Sakamoto H., Uda H. Human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction. Cancer. 1994 Jun 1;73(11):2740–2744. doi: 10.1002/1097-0142(19940601)73:11<2740::aid-cncr2820731115>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  24. Takahashi T., Takahashi T., Suzuki H., Hida T., Sekido Y., Ariyoshi Y., Ueda R. The p53 gene is very frequently mutated in small-cell lung cancer with a distinct nucleotide substitution pattern. Oncogene. 1991 Oct;6(10):1775–1778. [PubMed] [Google Scholar]
  25. Takebe N., Tsunokawa Y., Nozawa S., Terada M., Sugimura T. Conservation of E6 and E7 regions of human papillomavirus types 16 and 18 present in cervical cancers. Biochem Biophys Res Commun. 1987 Mar 30;143(3):837–844. doi: 10.1016/0006-291x(87)90325-1. [DOI] [PubMed] [Google Scholar]
  26. Tase T., Okagaki T., Clark B. A., Manias D. A., Ostrow R. S., Twiggs L. B., Faras A. J. Human papillomavirus types and localization in adenocarcinoma and adenosquamous carcinoma of the uterine cervix: a study by in situ DNA hybridization. Cancer Res. 1988 Feb 15;48(4):993–998. [PubMed] [Google Scholar]
  27. Werness B. A., Levine A. J., Howley P. M. Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science. 1990 Apr 6;248(4951):76–79. doi: 10.1126/science.2157286. [DOI] [PubMed] [Google Scholar]
  28. Willey J. C., Broussoud A., Sleemi A., Bennett W. P., Cerutti P., Harris C. C. Immortalization of normal human bronchial epithelial cells by human papillomaviruses 16 or 18. Cancer Res. 1991 Oct 1;51(19):5370–5377. [PubMed] [Google Scholar]
  29. Woodworth C. D., Bowden P. E., Doniger J., Pirisi L., Barnes W., Lancaster W. D., DiPaolo J. A. Characterization of normal human exocervical epithelial cells immortalized in vitro by papillomavirus types 16 and 18 DNA. Cancer Res. 1988 Aug 15;48(16):4620–4628. [PubMed] [Google Scholar]
  30. Yee C., Krishnan-Hewlett I., Baker C. C., Schlegel R., Howley P. M. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am J Pathol. 1985 Jun;119(3):361–366. [PMC free article] [PubMed] [Google Scholar]
  31. Yokota J., Akiyama T., Fung Y. K., Benedict W. F., Namba Y., Hanaoka M., Wada M., Terasaki T., Shimosato Y., Sugimura T. Altered expression of the retinoblastoma (RB) gene in small-cell carcinoma of the lung. Oncogene. 1988 Oct;3(4):471–475. [PubMed] [Google Scholar]
  32. Yousem S. A., Ohori N. P., Sonmez-Alpan E. Occurrence of human papillomavirus DNA in primary lung neoplasms. Cancer. 1992 Feb 1;69(3):693–697. doi: 10.1002/1097-0142(19920201)69:3<693::aid-cncr2820690316>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  33. de Villiers E. M. Heterogeneity of the human papillomavirus group. J Virol. 1989 Nov;63(11):4898–4903. doi: 10.1128/jvi.63.11.4898-4903.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. zur Hausen H. Viruses in human cancers. Science. 1991 Nov 22;254(5035):1167–1173. doi: 10.1126/science.1659743. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES