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. 1995 Apr;71(4):845–853. doi: 10.1038/bjc.1995.163

A new human breast cancer cell line, KPL-1 secretes tumour-associated antigens and grows rapidly in female athymic nude mice.

J Kurebayashi 1, M Kurosumi 1, H Sonoo 1
PMCID: PMC2033762  PMID: 7710953

Abstract

We recently established a new human breast cell line, designated KPL-1, which was derived from the malignant effusion of a patient with breast cancer. This cell line is highly tumorigenic and grows rapidly in female nude mice. Cytogenetic analysis indicated its human origin and revealed a hypertriploid modal number of chromosomes. Electron microscopic examination suggested that the KPL-1 cells are of epithelial origin. Immunohistochemical studies revealed that the cells express cytokeratin, carcinoembryonic antigen and CA 15-3. They also possess a large number of oestrogen receptors but not progesterone receptors. Interestingly, KPL-1 cells seem to grow oestrogen independently in vitro. No amplification of c-erbB-2, c-myc, H-ras and N-ras genes was detected. KPL-1 cells secrete a large amount of tissue polypeptide antigen (TPA). Although the secretion of CA 15-3 seemed to be constant throughout all cell growth phases, TPA secretion increased during the exponential growth phase and decreased during the plateau phase. Serum TPA levels significantly correlated with the volume of KPL-1 tumours transplanted into nude mice. These data suggest that this KPL-1 cell line may be useful for studying oestrogen-independent growth and the kinetics of tumour-associated antigens in vivo as well as in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Kufe D. W. Identification of a family of high molecular weight tumor-associated glycoproteins. J Immunol. 1987 Jul 1;139(1):257–261. [PubMed] [Google Scholar]
  2. Björklund B., Björklund V. Specificity and basis of the tissue polypeptide antigen. Cancer Detect Prev. 1983;6(1-2):41–50. [PubMed] [Google Scholar]
  3. Cailleau R., Young R., Olivé M., Reeves W. J., Jr Breast tumor cell lines from pleural effusions. J Natl Cancer Inst. 1974 Sep;53(3):661–674. doi: 10.1093/jnci/53.3.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chen L. C., Kurisu W., Ljung B. M., Goldman E. S., Moore D., 2nd, Smith H. S. Heterogeneity for allelic loss in human breast cancer. J Natl Cancer Inst. 1992 Apr 1;84(7):506–510. doi: 10.1093/jnci/84.7.506. [DOI] [PubMed] [Google Scholar]
  5. Clarke R., Brünner N., Katzenellenbogen B. S., Thompson E. W., Norman M. J., Koppi C., Paik S., Lippman M. E., Dickson R. B. Progression of human breast cancer cells from hormone-dependent to hormone-independent growth both in vitro and in vivo. Proc Natl Acad Sci U S A. 1989 May;86(10):3649–3653. doi: 10.1073/pnas.86.10.3649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Engel L. W., Young N. A., Tralka T. S., Lippman M. E., O'Brien S. J., Joyce M. J. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 1978 Oct;38(10):3352–3364. [PubMed] [Google Scholar]
  7. Fidler I. J. 7th Jan Waldenström Lecture. The biology of human cancer metastasis. Acta Oncol. 1991;30(6):668–675. doi: 10.3109/02841869109092438. [DOI] [PubMed] [Google Scholar]
  8. Fuqua S. A., Fitzgerald S. D., Allred D. C., Elledge R. M., Nawaz Z., McDonnell D. P., O'Malley B. W., Greene G. L., McGuire W. L. Inhibition of estrogen receptor action by a naturally occurring variant in human breast tumors. Cancer Res. 1992 Jan 15;52(2):483–486. [PubMed] [Google Scholar]
  9. Gion M., Mione R., Gatti C., Dittadi R., Leon A., Castiglioni C., Nascimben O., Bruscagnin G. Tissue polypeptide antigen in tumor cytosol: a new prognostic indicator in primary breast cancer. Breast Cancer Res Treat. 1990 Nov;17(1):15–21. doi: 10.1007/BF01812680. [DOI] [PubMed] [Google Scholar]
  10. Gion M., Mione R., Gatti C., Dittadi R., Leon A., Nascimben O., Pizzorno B., Bruscagnin G. Is tissue polypeptide antigen still a useful tumor marker in breast carcinoma? Comparison with CA15.3 and MCA. Tumori. 1990 Aug 31;76(4):360–364. doi: 10.1177/030089169007600411. [DOI] [PubMed] [Google Scholar]
  11. Gottardis M. M., Jordan V. C. Development of tamoxifen-stimulated growth of MCF-7 tumors in athymic mice after long-term antiestrogen administration. Cancer Res. 1988 Sep 15;48(18):5183–5187. [PubMed] [Google Scholar]
  12. Hayes D. F., Zurawski V. R., Jr, Kufe D. W. Comparison of circulating CA15-3 and carcinoembryonic antigen levels in patients with breast cancer. J Clin Oncol. 1986 Oct;4(10):1542–1550. doi: 10.1200/JCO.1986.4.10.1542. [DOI] [PubMed] [Google Scholar]
  13. Horwitz K. B. Cellular heterogeneity and mutant oestrogen receptors in hormone resistant breast cancer. Cancer Surv. 1992;14:41–54. [PubMed] [Google Scholar]
  14. Kufe D., Inghirami G., Abe M., Hayes D., Justi-Wheeler H., Schlom J. Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors. Hybridoma. 1984 Fall;3(3):223–232. doi: 10.1089/hyb.1984.3.223. [DOI] [PubMed] [Google Scholar]
  15. Kurebayashi J., Ishida T., Higashi Y., Suemasu K., Nomoto C., Yoshida K. Prognostic significances of estrogen and progesterone receptors in primary operable breast cancer. Jpn J Clin Oncol. 1990 Sep;20(3):271–280. [PubMed] [Google Scholar]
  16. Lüning B., Wiklund B., Redelius P., Björklund B. Biochemical properties of tissue polypeptide antigen. Biochim Biophys Acta. 1980 Jul 24;624(1):90–101. doi: 10.1016/0005-2795(80)90228-7. [DOI] [PubMed] [Google Scholar]
  17. McLeskey S. W., Kurebayashi J., Honig S. F., Zwiebel J., Lippman M. E., Dickson R. B., Kern F. G. Fibroblast growth factor 4 transfection of MCF-7 cells produces cell lines that are tumorigenic and metastatic in ovariectomized or tamoxifen-treated athymic nude mice. Cancer Res. 1993 May 1;53(9):2168–2177. [PubMed] [Google Scholar]
  18. Mross K., Bandlow G. The clinical significance of tissue polypeptide antigen in breast cancer patients. Anticancer Res. 1986 Mar-Apr;6(2):223–225. [PubMed] [Google Scholar]
  19. Murphy L. C., Dotzlaw H. Variant estrogen receptor mRNA species detected in human breast cancer biopsy samples. Mol Endocrinol. 1989 Apr;3(4):687–693. doi: 10.1210/mend-3-4-687. [DOI] [PubMed] [Google Scholar]
  20. Nemoto T., Constantine R., Chu T. M. Human tissue polypeptide antigen in breast cancer. J Natl Cancer Inst. 1979 Dec;63(6):1347–1350. [PubMed] [Google Scholar]
  21. Ozzello L., Sordat M. Behavior of tumors produced by transplantation of human mammary cell lines in athymic nude mice. Eur J Cancer. 1980 Apr;16(4):553–559. doi: 10.1016/0014-2964(80)90235-2. [DOI] [PubMed] [Google Scholar]
  22. Price J. E., Polyzos A., Zhang R. D., Daniels L. M. Tumorigenicity and metastasis of human breast carcinoma cell lines in nude mice. Cancer Res. 1990 Feb 1;50(3):717–721. [PubMed] [Google Scholar]
  23. Scholl S. M., Huff K. K., Lippman M. E. Antiestrogenic effects of LY 117018 in MCF-7 cells. Endocrinology. 1983 Aug;113(2):611–617. doi: 10.1210/endo-113-2-611. [DOI] [PubMed] [Google Scholar]
  24. Shafie S. M., Liotta L. A. Formation of metastasis by human breast carcinoma cells (MCF-7) in nude mice. Cancer Lett. 1980 Dec;11(2):81–87. doi: 10.1016/0304-3835(80)90097-x. [DOI] [PubMed] [Google Scholar]
  25. Soule H. D., McGrath C. M. Estrogen responsive proliferation of clonal human breast carcinoma cells in athymic mice. Cancer Lett. 1980 Aug;10(2):177–189. doi: 10.1016/0304-3835(80)90042-7. [DOI] [PubMed] [Google Scholar]
  26. Soule H. D., Vazguez J., Long A., Albert S., Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. doi: 10.1093/jnci/51.5.1409. [DOI] [PubMed] [Google Scholar]
  27. Wang Y., Miksicek R. J. Identification of a dominant negative form of the human estrogen receptor. Mol Endocrinol. 1991 Nov;5(11):1707–1715. doi: 10.1210/mend-5-11-1707. [DOI] [PubMed] [Google Scholar]
  28. Weber K., Osborn M., Moll R., Wiklund B., Lüning B. Tissue polypeptide antigen (TPA) is related to the non-epidermal keratins 8, 18 and 19 typical of simple and non-squamous epithelia: re-evaluation of a human tumor marker. EMBO J. 1984 Nov;3(11):2707–2714. doi: 10.1002/j.1460-2075.1984.tb02198.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wolman S. R., Dawson P. J. Genetic events in breast cancer and their clinical correlates. Crit Rev Oncog. 1991;2(4):277–291. [PubMed] [Google Scholar]
  30. Yasui W., Sumiyoshi H., Hata J., Kameda T., Ochiai A., Ito H., Tahara E. Expression of epidermal growth factor receptor in human gastric and colonic carcinomas. Cancer Res. 1988 Jan 1;48(1):137–141. [PubMed] [Google Scholar]

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