Thr38 and Ser198 are Pto autophosphorylation sites required for the AvrPto–Pto-mediated hypersensitive response. Sessa Guido, D’Ascenzo Mark, B.Martin Gregory. The EMBO Journal. 2000;19:2257–2269. doi: 10.1093/emboj/19.10.2257.
The publishers apologize for errors printed in Table I. Table I is shown correctly below.
Table I. Phosphopeptides identified in a tryptic digest of in vitro autophosphorylated GST–Pto.
| Peaka | P-AAb | Determined by | Sequence | No. of P-groups | Site(s) identified |
|---|---|---|---|---|---|
| A and B | P-Thr | Phosphopeptide map of Pto(T288A) mutant | 285FGDTAVK291 | 1 | Thr288 |
| 284KFGDTAVK291 | |||||
| C | ND | ||||
| D | MALDI-TOF/MS | 189GTELDQTHLSTVVK202 | 1, 2 or 3 | ND | |
| 188KGTELDQTHLSTVVK202 | 1, 2 or 3 | ||||
| E | MALDI-TOF/MS | 189GTELDQTHLSTVVK202 | 1, 2 or 4 | Thr190, Thr195, Ser198, Thr199 | |
| F | P-Thr | MALDI-TOF/MS | 29VPLVDLEEATNNFDHK44 | 1 | Thr38 |
| G | P-Thr | MALDI-TOF/MS, automated | 29VPLVDLEEATNNFDHK44 | 1 | Thr38 |
| Edman degradation | |||||
| H | P-Thr | MALDI-TOF/MS | 125HLYGSDLPTMSMSWEQR141 | 1 | Thr133 |
| I | P-Ser | MALDI-TOF/MS, manual | 8ATNSINDALSSSYLVPFESYR28 | 1 | Ser17 |
| Edman degradation | |||||
| J | ND | ||||
| K | ND |
aRadioactive peaks detected in HPLC fractionation of a tryptic digest of autophosphorylated Pto, as indicated in Figure 1.
bPhosphoamino acids determined as described in Materials and methods.
ND, not determined.