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. 1995 May;71(5):1081–1086. doi: 10.1038/bjc.1995.209

Evidence for a weak angiogenic response to human colorectal cancers.

A J Pritchard 1, T Chatterjee 1, M Wilkinson 1, D G Powe 1, T Gray 1, R E Hewitt 1
PMCID: PMC2033771  PMID: 7537517

Abstract

Many previous qualitative studies have shown that tumours are less vascular in the centre, and that host tissues become more vascular in close proximity to tumours. However, quantitative findings presented here for human colorectal cancer reveal some significant differences. Sections from 20 colorectal carcinomas (ten moderately and ten poorly differentiated) were immunostained with the QB/end/10 monoclonal to demonstrate blood vessels. These were measured by interactive morphometry and vascular volume density, surface density (Sv) and length density were recorded. In poorly differentiated carcinomas, the tumour centre was significantly less vascular than the periphery for all three parameters (P = 0.008 for Sv). However, no significant difference was seen for moderately differentiated tumours, which constitute the majority of colorectal cancers. Surrounding host tissues did not show a general increase in vascular density close to tumours. Furthermore, when total viable tissue was considered, the vascular density of carcinomas was not markedly different from normal mucosa. In the centre of moderately differentiated carcinomas for example, the mean value for Sv was only 1.4 times higher than the mean value for normal mucosa. These findings suggest that colorectal cancers may elicit a relatively weak angiogenic response, consistent with their exceptionally slow growth rate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anthony P. P., Ramani P. Endothelial markers in malignant vascular tumours of the liver: superiority of QB-END/10 over von Willebrand factor and Ulex europaeus agglutinin 1. J Clin Pathol. 1991 Jan;44(1):29–32. doi: 10.1136/jcp.44.1.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carnochan P., Briggs J. C., Westbury G., Davies A. J. The vascularity of cutaneous melanoma: a quantitative histological study of lesions 0.85-1.25 mm in thickness. Br J Cancer. 1991 Jul;64(1):102–107. doi: 10.1038/bjc.1991.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Folkman J. Tumor angiogenesis. Adv Cancer Res. 1985;43:175–203. doi: 10.1016/s0065-230x(08)60946-x. [DOI] [PubMed] [Google Scholar]
  4. GOLDACRE R. J., SYLVEN B. On the access of blood-borne dyes to various tumour regions. Br J Cancer. 1962 Jun;16:306–322. doi: 10.1038/bjc.1962.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gabbert H., Wagner R., Höhn P. The relation between tumor cell proliferation and vascularization in differentiated and undifferentiated colon carcinomas in the rat. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982;41(1-2):119–131. doi: 10.1007/BF02890276. [DOI] [PubMed] [Google Scholar]
  6. Goldmann E. The Growth of Malignant Disease in Man and the Lower Animals, with special reference to the Vascular System. Proc R Soc Med. 1908;1(SURG):1–13. doi: 10.1177/003591570800101201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hewitt R. E., Powe D. G., Carter G. I., Turner D. R., Price J. E. Basement membrane collagen-IV synthesis in colorectal tumours. Int J Cancer. 1992 Jun 19;51(4):530–536. doi: 10.1002/ijc.2910510405. [DOI] [PubMed] [Google Scholar]
  8. Hewitt R. E., Powe D. G., Griffin N. R., Turner D. R. Relationships between epithelial basement membrane staining patterns in primary colorectal carcinomas and the extent of tumour spread. Int J Cancer. 1991 Jul 30;48(6):855–860. doi: 10.1002/ijc.2910480611. [DOI] [PubMed] [Google Scholar]
  9. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  10. OWEN L. N. A rapid method for studying tumour blood supply using lissamine green. Nature. 1960 Aug 27;187:795–796. doi: 10.1038/187795a0. [DOI] [PubMed] [Google Scholar]
  11. Parums D. V., Cordell J. L., Micklem K., Heryet A. R., Gatter K. C., Mason D. Y. JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol. 1990 Sep;43(9):752–757. doi: 10.1136/jcp.43.9.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Porschen R., Classen S., Piontek M., Borchard F. Vascularization of carcinomas of the esophagus and its correlation with tumor proliferation. Cancer Res. 1994 Jan 15;54(2):587–591. [PubMed] [Google Scholar]
  13. Ramani P., Bradley N. J., Fletcher C. D. QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathology. 1990 Sep;17(3):237–242. doi: 10.1111/j.1365-2559.1990.tb00713.x. [DOI] [PubMed] [Google Scholar]
  14. Srivastava A., Laidler P., Davies R. P., Horgan K., Hughes L. E. The prognostic significance of tumor vascularity in intermediate-thickness (0.76-4.0 mm thick) skin melanoma. A quantitative histologic study. Am J Pathol. 1988 Nov;133(2):419–423. [PMC free article] [PubMed] [Google Scholar]
  15. Srivastava A., Laidler P., Hughes L. E., Woodcock J., Shedden E. J. Neovascularization in human cutaneous melanoma: a quantitative morphological and Doppler ultrasound study. Eur J Cancer Clin Oncol. 1986 Oct;22(10):1205–1209. doi: 10.1016/0277-5379(86)90322-6. [DOI] [PubMed] [Google Scholar]
  16. Thompson W. D., Shiach K. J., Fraser R. A., McIntosh L. C., Simpson J. G. Tumours acquire their vasculature by vessel incorporation, not vessel ingrowth. J Pathol. 1987 Apr;151(4):323–332. doi: 10.1002/path.1711510413. [DOI] [PubMed] [Google Scholar]
  17. Warren B. A. The ultrastructure of the microcirculation at the advancing edge of Walker 256 carcinoma. Microvasc Res. 1970 Oct;2(4):443–453. doi: 10.1016/0026-2862(70)90037-3. [DOI] [PubMed] [Google Scholar]
  18. Weidner N., Carroll P. R., Flax J., Blumenfeld W., Folkman J. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol. 1993 Aug;143(2):401–409. [PMC free article] [PubMed] [Google Scholar]
  19. Weidner N., Folkman J., Pozza F., Bevilacqua P., Allred E. N., Moore D. H., Meli S., Gasparini G. Tumor angiogenesis: a new significant and independent prognostic indicator in early-stage breast carcinoma. J Natl Cancer Inst. 1992 Dec 16;84(24):1875–1887. doi: 10.1093/jnci/84.24.1875. [DOI] [PubMed] [Google Scholar]

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