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. 1995 Sep;72(3):620–626. doi: 10.1038/bjc.1995.383

Heat shock protein expression in testis and bladder cancer cell lines exhibiting differential sensitivity to heat.

E H Richards 1, J A Hickman 1, J R Masters 1
PMCID: PMC2033863  PMID: 7669571

Abstract

Testis cancer cells are more sensitive than bladder and most other cancer cells to chemotherapeutic drugs both in the clinic and in vitro. In this study we show that they are also more sensitive than bladder cancer cells to heat. Since heat and drug sensitivity may be related to the ability of a cell to mount a stress response, constitutive and induced levels of heat shock proteins (HSPs) in three testis and three bladder human cancer cell lines were measured using Western blotting and scanning densitometry. No correlation between constitutive levels of HSP 90 or HSP 73/72 and cellular heat sensitivity was found. However, HSP 27 levels were much lower in the testis tumour cells, suggesting that low HSP 27 expression might contribute to heat sensitivity. Protein synthesis studies using [35S]methionine indicated that, for the same heat shocks, the kinetics of synthesis and decay of HSP 90 and HSP 73/72 in 833K (the most heat sensitive testis cells) was similar to or greater than that in HT1376 (the most heat-resistant bladder cells). Both 833K and HT1376 developed thermotolerance, and this followed an increase in synthesis of HSPs. These results indicate that, although there are differences in the constitutive levels of HSPs between testis and bladder cancer cells, both cell types are capable of mounting an induced heat shock response and can develop a similar degree of thermotolerance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bedford P., Fichtinger-Schepman A. M., Shellard S. A., Walker M. C., Masters J. R., Hill B. T. Differential repair of platinum-DNA adducts in human bladder and testicular tumor continuous cell lines. Cancer Res. 1988 Jun 1;48(11):3019–3024. [PubMed] [Google Scholar]
  2. Bronson D. L., Andrews P. W., Solter D., Cervenka J., Lange P. H., Fraley E. E. Cell line derived from a metastasis of a human testicular germ cell tumor. Cancer Res. 1980 Jul;40(7):2500–2506. [PubMed] [Google Scholar]
  3. Bubeník J., Baresová M., Viklický V., Jakoubková J., Sainerová H., Donner J. Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int J Cancer. 1973 May;11(3):765–773. doi: 10.1002/ijc.2910110327. [DOI] [PubMed] [Google Scholar]
  4. Chrétien P., Landry J. Enhanced constitutive expression of the 27-kDa heat shock proteins in heat-resistant variants from Chinese hamster cells. J Cell Physiol. 1988 Oct;137(1):157–166. doi: 10.1002/jcp.1041370119. [DOI] [PubMed] [Google Scholar]
  5. Ciocca D. R., Fuqua S. A., Lock-Lim S., Toft D. O., Welch W. J., McGuire W. L. Response of human breast cancer cells to heat shock and chemotherapeutic drugs. Cancer Res. 1992 Jul 1;52(13):3648–3654. [PubMed] [Google Scholar]
  6. Craig E. A. The heat shock response. CRC Crit Rev Biochem. 1985;18(3):239–280. doi: 10.3109/10409238509085135. [DOI] [PubMed] [Google Scholar]
  7. Crête P., Landry J. Induction of HSP27 phosphorylation and thermoresistance in Chinese hamster cells by arsenite, cycloheximide, A23187, and EGTA. Radiat Res. 1990 Mar;121(3):320–327. [PubMed] [Google Scholar]
  8. Fry A. M., Chresta C. M., Davies S. M., Walker M. C., Harris A. L., Hartley J. A., Masters J. R., Hickson I. D. Relationship between topoisomerase II level and chemosensitivity in human tumor cell lines. Cancer Res. 1991 Dec 15;51(24):6592–6595. [PubMed] [Google Scholar]
  9. Huot J., Roy G., Lambert H., Chrétien P., Landry J. Increased survival after treatments with anticancer agents of Chinese hamster cells expressing the human Mr 27,000 heat shock protein. Cancer Res. 1991 Oct 1;51(19):5245–5252. [PubMed] [Google Scholar]
  10. Kampinga H. H. Thermotolerance in mammalian cells. Protein denaturation and aggregation, and stress proteins. J Cell Sci. 1993 Jan;104(Pt 1):11–17. doi: 10.1242/jcs.104.1.11. [DOI] [PubMed] [Google Scholar]
  11. Landry J., Chrétien P., Lambert H., Hickey E., Weber L. A. Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells. J Cell Biol. 1989 Jul;109(1):7–15. doi: 10.1083/jcb.109.1.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lavoie J. N., Gingras-Breton G., Tanguay R. M., Landry J. Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization. J Biol Chem. 1993 Feb 15;268(5):3420–3429. [PubMed] [Google Scholar]
  13. Lee W. C., Lin K. Y., Chen K. D., Lai Y. K. Induction of HSP70 is associated with vincristine resistance in heat-shocked 9L rat brain tumour cells. Br J Cancer. 1992 Oct;66(4):653–659. doi: 10.1038/bjc.1992.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Li G. C. Elevated levels of 70,000 dalton heat shock protein in transiently thermotolerant Chinese hamster fibroblasts and in their stable heat resistant variants. Int J Radiat Oncol Biol Phys. 1985 Jan;11(1):165–177. doi: 10.1016/0360-3016(85)90376-1. [DOI] [PubMed] [Google Scholar]
  15. Li G. C., Werb Z. Correlation between synthesis of heat shock proteins and development of thermotolerance in Chinese hamster fibroblasts. Proc Natl Acad Sci U S A. 1982 May;79(10):3218–3222. doi: 10.1073/pnas.79.10.3218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lindquist S., Craig E. A. The heat-shock proteins. Annu Rev Genet. 1988;22:631–677. doi: 10.1146/annurev.ge.22.120188.003215. [DOI] [PubMed] [Google Scholar]
  17. Löwer J., Löwer R., Stegmann J., Frank H., Kurth R. Retrovirus particle production in three of four human teratocarcinoma cell lines. Haematol Blood Transfus. 1981;26:541–544. doi: 10.1007/978-3-642-67984-1_101. [DOI] [PubMed] [Google Scholar]
  18. Masters J. R., Hepburn P. J., Walker L., Highman W. J., Trejdosiewicz L. K., Povey S., Parkar M., Hill B. T., Riddle P. R., Franks L. M. Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines. Cancer Res. 1986 Jul;46(7):3630–3636. [PubMed] [Google Scholar]
  19. Masters J. R., Osborne E. J., Walker M. C., Parris C. N. Hypersensitivity of human testis-tumour cell lines to chemotherapeutic drugs. Int J Cancer. 1993 Jan 21;53(2):340–346. doi: 10.1002/ijc.2910530228. [DOI] [PubMed] [Google Scholar]
  20. Oesterreich S., Weng C. N., Qiu M., Hilsenbeck S. G., Osborne C. K., Fuqua S. A. The small heat shock protein hsp27 is correlated with growth and drug resistance in human breast cancer cell lines. Cancer Res. 1993 Oct 1;53(19):4443–4448. [PubMed] [Google Scholar]
  21. Pera M. F., Friedlos F., Mills J., Roberts J. J. Inherent sensitivity of cultured human embryonal carcinoma cells to adducts of cis-diamminedichloroplatinum(II) on DNA. Cancer Res. 1987 Dec 15;47(24 Pt 1):6810–6813. [PubMed] [Google Scholar]
  22. Rasheed S., Gardner M. B., Rongey R. W., Nelson-Rees W. A., Arnstein P. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J Natl Cancer Inst. 1977 Apr;58(4):881–890. doi: 10.1093/jnci/58.4.881. [DOI] [PubMed] [Google Scholar]
  23. Seidman A. D., Scher H. I. The evolving role of chemotherapy for muscle infiltrating bladder cancer. Semin Oncol. 1991 Dec;18(6):585–595. [PubMed] [Google Scholar]
  24. Tannock I., Gospodarowicz M., Connolly J., Jewett M. M-VAC (methotrexate, vinblastine, doxorubicin and cisplatin) chemotherapy for transitional cell carcinoma: the Princess Margaret Hospital experience. J Urol. 1989 Aug;142(2 Pt 1):289–292. doi: 10.1016/s0022-5347(17)38733-5. [DOI] [PubMed] [Google Scholar]
  25. Walker M. C., Parris C. N., Masters J. R. Differential sensitivities of human testicular and bladder tumor cell lines to chemotherapeutic drugs. J Natl Cancer Inst. 1987 Aug;79(2):213–216. [PubMed] [Google Scholar]
  26. Welch W. J. Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev. 1992 Oct;72(4):1063–1081. doi: 10.1152/physrev.1992.72.4.1063. [DOI] [PubMed] [Google Scholar]

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