Table 2.
Kinetic Parameter Estimates for Binding of InlB and InlB-ΔBΔGW1 to Heparin with the Two-Site Heterogeneous Ligand Model for Each Set of Dataa
| average InlB | average InlB-ΔBΔGW1 | |
|---|---|---|
| kt (M-1 s-1) | 1.5 × 109 ± 0.3 | 5 × 108 ± 1 |
| site 1 | ||
| ka1 (M-1 s-1) | 3.2 × 106 ± 0.6 | 1.2 × 106 ± 0.5 |
| kd1 (s-1) | 2 × 10-3 ± 0.6 | 1.2 × 10-3 ± 0.2 |
| Rmax1 (RU) | 46 ± 5 | 29 ± 7 |
| KD1 (M-1) | 6.4 × 10-10 ± 0.8 | 1.1 × 10-9 ± 0.5 |
| site 2 | ||
| ka2 (M-1 s-1) | 1 × 108 ± 0.1 | 17 × 107 ± 24 |
| kd2 (s-1) | 0.5 ± 0.1 | 2 ± 2 |
| Rmax2 (RU) | 440 ± 41 | 350 ± 23 |
| KD2 (M-1) | 5 × 10-9 ± 1 | 1.5 × 10-8 ± 0.4 |
| χ2 | 4.9 ± 3.7 | 2.9 ± 0.7 |
a
The average was calculated on the basis of the kinetic data obtained from three independent sets of experiments (data not shown).