Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Jul;52(1):197–199. doi: 10.1128/aem.52.1.197-199.1986

Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

I Roberts, W M Holmes, P B Hylemon
PMCID: PMC203446  PMID: 2873789

Abstract

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.

Full text

PDF
197

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaschek H. P., Klacik M. A. Role of DNase in recovery of plasmid DNA from Clostridium perfringens. Appl Environ Microbiol. 1984 Jul;48(1):178–181. doi: 10.1128/aem.48.1.178-181.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blaschek H. P., Solberg M. Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B. J Bacteriol. 1981 Jul;147(1):262–266. doi: 10.1128/jb.147.1.262-266.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Currier T. C., Nester E. W. Isolation of covalently closed circular DNA of high molecular weight from bacteria. Anal Biochem. 1976 Dec;76(2):431–441. doi: 10.1016/0003-2697(76)90338-9. [DOI] [PubMed] [Google Scholar]
  6. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rood J. I., Maher E. A., Somers E. B., Campos E., Duncan C. L. Isolation and characterization of multiply antibiotic-resistant Clostridum perfringens strains from porcine feces. Antimicrob Agents Chemother. 1978 May;13(5):871–880. doi: 10.1128/aac.13.5.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Squires C. H., Heefner D. L., Evans R. J., Kopp B. J., Yarus M. J. Shuttle plasmids for Escherichia coli and Clostridium perfringens. J Bacteriol. 1984 Aug;159(2):465–471. doi: 10.1128/jb.159.2.465-471.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES