Figure 3.—

Recombination breakpoints of the three most informative recombinants between Mel6 and TW1. The recombinants were screened from a laboratory population that experienced over 20 generations of recombination. These third chromosome recombinants had the X and the second chromosomes from Mel6. Horizontal arrows indicate annotated ORFs with their transcriptional directions. Vertical arrowheads indicate positions of the markers genotyped. Open and solid bars represent chromosome segments from Mel6 and TW1, respectively. Shaded bars indicate regions where recombinant breakpoints reside. The markers were genotyped by directly sequencing PCR fragments of ∼600–900 bp. The thoracic tridents of the three recombinants were all pigmented. Note that the critical interval of ∼75kb between CG7000 and CG5892 includes the candidate gene ebony.