Abstract
Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. The ability to hydrolyze xylan was generally uncoupled from that to hydrolyze cellulose; only three of the xylan-positive strains also yielded a positive test for cellulolytic activity. Of the 19 xylanolytic strains, 2, P. stipitis CBS 5773 and CBS 5775, converted xylan into ethanol, with about 60% of a theoretical yield computed on the basis of the amount of d-xylose present originally that could be released by acid hydrolysis.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biely P., Krátký Z., Kocková-Kratochvílová A., Bauer S. Xylan-degrading activity in yeasts: growth on xylose, xylan and hemicelluloses. Folia Microbiol (Praha) 1978;23(5):366–371. doi: 10.1007/BF02876436. [DOI] [PubMed] [Google Scholar]
- Biely P., Mislovicová D., Toman R. Soluble chromogenic substrates for the assay of endo-1,4-beta-xylanases and endo-1,4-beta-glucanases. Anal Biochem. 1985 Jan;144(1):142–146. doi: 10.1016/0003-2697(85)90095-8. [DOI] [PubMed] [Google Scholar]
- Maleszka R., Schneider H. Fermentation of D-xylose, xylitol, and D-xylulose by yeasts. Can J Microbiol. 1982 Mar;28(3):360–363. doi: 10.1139/m82-054. [DOI] [PubMed] [Google Scholar]
- TIMELL T. E. WOOD HEMICELLULOSES. I. Adv Carbohydr Chem. 1964;19:247–302. [PubMed] [Google Scholar]
- Toivola A., Yarrow D., van den Bosch E., van Dijken J. P., Scheffers W. A. Alcoholic Fermentation of d-Xylose by Yeasts. Appl Environ Microbiol. 1984 Jun;47(6):1221–1223. doi: 10.1128/aem.47.6.1221-1223.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]