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. 1986 Aug;52(2):366–370. doi: 10.1128/aem.52.2.366-370.1986

Transfer of Plasmids to an Antibiotic-Sensitive Mutant of Zymomonas mobilis†

Steven E Buchholz 1,*, Douglas E Eveleigh 1
PMCID: PMC203531  PMID: 16347136

Abstract

Wild-type strains of Zymomonas mobilis exhibit multiple antibiotic resistance and thus restrict the use of many broad-host-range plasmids in them as cloning vehicles. Antibiotic-sensitive mutants of Z. mobilis were isolated and used as hosts for the conjugal transfer of broad-host-range plasmids from Escherichia coli. Such antibiotic-sensitive strains can facilitate the application of broad-host-range plasmids to the study of Z. mobilis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagdasarian M., Timmis K. N. Host: vector systems for gene cloning in Pseudomonas. Curr Top Microbiol Immunol. 1982;96:47–67. doi: 10.1007/978-3-642-68315-2_4. [DOI] [PubMed] [Google Scholar]
  2. Cornelis G., Ghosal D., Saedler H. Tn951: a new transposon carrying a lactose operon. Mol Gen Genet. 1978 Apr 6;160(2):215–224. doi: 10.1007/BF00267484. [DOI] [PubMed] [Google Scholar]
  3. Ditta G., Stanfield S., Corbin D., Helinski D. R. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. doi: 10.1073/pnas.77.12.7347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goodman A. E., Rogers P. L., Skotnicki M. L. Minimal medium for isolation of auxotrophic Zymomonas mutants. Appl Environ Microbiol. 1982 Aug;44(2):496–498. doi: 10.1128/aem.44.2.496-498.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Haffie T. L., Louie P. W., Khachatourians G. G. Isolation of Noninhibitory Strains of Zymomonas mobilis. Appl Environ Microbiol. 1985 Apr;49(4):1007–1009. doi: 10.1128/aem.49.4.1007-1009.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  7. Holloway B. W. Plasmids that mobilize bacterial chromosome. Plasmid. 1979 Jan;2(1):1–19. doi: 10.1016/0147-619x(79)90002-7. [DOI] [PubMed] [Google Scholar]
  8. Skotnicki M. L., Tribe D. E., Rogers P. L. R-Plasmid Transfer in Zymomonas mobilis. Appl Environ Microbiol. 1980 Jul;40(1):7–12. doi: 10.1128/aem.40.1.7-12.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stokes H. W., Dally E. L., Yablonsky M. D., Eveleigh D. E. Comparison of plasmids in strains of Zymomonas mobilis. Plasmid. 1983 Mar;9(2):138–146. doi: 10.1016/0147-619x(83)90016-1. [DOI] [PubMed] [Google Scholar]
  10. Swings J., De Ley J. The biology of Zymomonas. Bacteriol Rev. 1977 Mar;41(1):1–46. doi: 10.1128/br.41.1.1-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Walia S. K., Carey V. C., All B. P., 3rd, Ingram L. O. Self-transmissible plasmid in Zymomonas mobilis carrying antibiotic resistance. Appl Environ Microbiol. 1984 Jan;47(1):198–200. doi: 10.1128/aem.47.1.198-200.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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