Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1986 Sep;52(3):430–433. doi: 10.1128/aem.52.3.430-433.1986

Practical direct plaque assay for coliphages in 100-ml samples of drinking water.

W O Grabow, P Coubrough
PMCID: PMC203551  PMID: 3532952

Abstract

A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.

Full text

PDF
430

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Farber F. E., Gradwohl S. E., Sanford P. B., Tobin M. J., Lee K. J., Gerba C. P. Bacteriophage concentration from water by filter chromatography. J Virol Methods. 1983 Dec;7(5-6):297–304. doi: 10.1016/0166-0934(83)90082-4. [DOI] [PubMed] [Google Scholar]
  2. Havelaar A. H., Hogeboom W. M. Factors affecting the enumeration of coliphages in sewage and sewage-polluted waters. Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):387–397. doi: 10.1007/BF00399318. [DOI] [PubMed] [Google Scholar]
  3. Isbister J. D., Simmons J. A., Scott W. M., Kitchens J. F. A simplified method for coliphage detection in natural waters. Acta Microbiol Pol. 1983;32(2):197–206. [PubMed] [Google Scholar]
  4. Kenard R. P., Valentine R. S. Rapid determination of the presence of enteric bacteria in water. Appl Microbiol. 1974 Mar;27(3):484–487. doi: 10.1128/am.27.3.484-487.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kennedy J. E., Jr, Bitton G., Oblinger J. L. Comparison of selective media for assay of coliphages in sewage effluent and lake water. Appl Environ Microbiol. 1985 Jan;49(1):33–36. doi: 10.1128/aem.49.1.33-36.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kott Y. Estimation of low numbers of Escherichia coli bacteriophage by use of the most probable number method. Appl Microbiol. 1966 Mar;14(2):141–144. doi: 10.1128/am.14.2.141-144.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Purdy R. N., Dancer B. N., Day M. J., Stickler D. J. A note on a membrane filtration method for the concentration and enumeration of bacteriophages from water. J Appl Bacteriol. 1985 Feb;58(2):231–233. doi: 10.1111/j.1365-2672.1985.tb01452.x. [DOI] [PubMed] [Google Scholar]
  8. Schulze E., Lenk J. Concentration of coliphages from drinking water by Mg(OH)2 flocculation. Naturwissenschaften. 1983 Dec;70(12):612–613. doi: 10.1007/BF00377405. [DOI] [PubMed] [Google Scholar]
  9. Seeley N. D., Primrose S. B. The isolation of bacteriophages from the environment. J Appl Bacteriol. 1982 Aug;53(1):1–17. doi: 10.1111/j.1365-2672.1982.tb04729.x. [DOI] [PubMed] [Google Scholar]
  10. Simková A., Cervenka J. Coliphages as ecological indicators of enteroviruses in various water systems. Bull World Health Organ. 1981;59(4):611–618. [PMC free article] [PubMed] [Google Scholar]
  11. Singh S. N., Gerba C. P. Concentration of coliphage from water and sewage with charge-modified filter aid. Appl Environ Microbiol. 1983 Jan;45(1):232–237. doi: 10.1128/aem.45.1.232-237.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wentsel R. S., O'Neill P. E., Kitchens J. F. Evaluation of coliphage detection as a rapid indicator of water quality. Appl Environ Microbiol. 1982 Feb;43(2):430–434. doi: 10.1128/aem.43.2.430-434.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yates M. V., Gerba C. P., Kelley L. M. Virus persistence in groundwater. Appl Environ Microbiol. 1985 Apr;49(4):778–781. doi: 10.1128/aem.49.4.778-781.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES