Abstract
Wild-type Aspergillus parasiticus produces, in addition to the colorless aflatoxins, a number of pigmented secondary metabolites. Examination of these pigments demonstrated that a major component was an anthraquinone, averufanin. Radiolabeling studies with [14C]averufanin showed that 23% of the label was incorporated into aflatoxin B1 by the wild type and that 31% of the label was incorporated into O-methylsterigmatocystin by a non-aflatoxin-producing isolate. In similar studies with blocked mutants of A. parasiticus the 14C label from averufanin was accumulated in averufin (72%) and versicolorin A (54%) but not averantin. The results demonstrate that averufanin is a biosynthetic precursor of aflatoxin B1 between averantin and averufin.
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- ADYE J., MATELES R. I. INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS. Biochim Biophys Acta. 1964 May 11;86:418–420. doi: 10.1016/0304-4165(64)90077-7. [DOI] [PubMed] [Google Scholar]
- Bennett J. W., Christensen S. B. New perspectives on aflatoxin biosynthesis. Adv Appl Microbiol. 1983;29:53–92. doi: 10.1016/s0065-2164(08)70354-x. [DOI] [PubMed] [Google Scholar]
- Bennett J. W., Fernholz F. A., Lee L. S. Effect of light on aflatoxins, anthraquinones, and sclerotia in Aspergillus flavus and A parasiticus. Mycologia. 1978 Jan-Feb;70(1):104–116. [PubMed] [Google Scholar]
- Bennett J. W., Lee L. S., Shoss S. M., Boudreaux G. H. Identification of averantin as an aflatoxin B1 precursor: placement in the biosynthetic pathway. Appl Environ Microbiol. 1980 Apr;39(4):835–839. doi: 10.1128/aem.39.4.835-839.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donkersloot J. A., Mateles R. I., Yang S. S. Isolation of averufin from a mutant of Aspergillus parasiticus impaired in aflatoxin biosynthesis. Biochem Biophys Res Commun. 1972 Jun 9;47(5):1051–1055. doi: 10.1016/0006-291x(72)90939-4. [DOI] [PubMed] [Google Scholar]
- Dutton M. F., Ehrlich K., Bennett J. W. Biosynthetic relationship among aflatoxins B1, B2, M1, and M2. Appl Environ Microbiol. 1985 Jun;49(6):1392–1395. doi: 10.1128/aem.49.6.1392-1395.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsieh D. P., Lin M. T., Yao R. C., Singh R. Biosynthesis of aflatoxin. Conversion of norsolorinic acid and other hypothetical intermediates into aflatoxin B1. J Agric Food Chem. 1976 Nov-Dec;24(6):1170–1174. doi: 10.1021/jf60208a018. [DOI] [PubMed] [Google Scholar]
- Lee L. S., Bennett J. W., Cucullu A. F., Ory R. L. Biosynthesis of aflatoxin B1. Conversion of versicolorin A to aflatoxin B1 by Aspergillus parasiticus. J Agric Food Chem. 1976 Nov-Dec;24(6):1167–1170. doi: 10.1021/jf60208a017. [DOI] [PubMed] [Google Scholar]
- Lee L. S., Bennett J. W., Cucullu A. F., Stanley J. B. Synthesis of versicolorin A by a mutant strain of Aspergillus parasiticus deficient in aflatoxin production. J Agric Food Chem. 1975 Nov-Dec;23(6):1132–1134. doi: 10.1021/jf60202a011. [DOI] [PubMed] [Google Scholar]
- Lee L., Bennett J. W., Goldblatt L. A., Lundin R. E. Norsolorinic acid from a mutant strain of Aspergillus parasiticus. J Am Oil Chem Soc. 1971 Feb;48(2):93–94. doi: 10.1007/BF02635696. [DOI] [PubMed] [Google Scholar]
- Lin M. T., Hsieh D. P. Averufin in the biosynthesis of aflatoxin B. J Am Chem Soc. 1973 Mar 7;95(5):1668–1669. doi: 10.1021/ja00786a056. [DOI] [PubMed] [Google Scholar]
- Lin M. T., Hsieh D. P., Yao R. C., Donkersloot J. A. Conversion of averufin into aflatoxins by Aspergillus parasiticus. Biochemistry. 1973 Dec 4;12(25):5167–5171. doi: 10.1021/bi00749a023. [DOI] [PubMed] [Google Scholar]
- Yao R. C., Hsieh D. P. Step of dichlorvos inhibition in the pathway of aflatoxin biosynthesis. Appl Microbiol. 1974 Jul;28(1):52–57. doi: 10.1128/am.28.1.52-57.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]