Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Jan;53(1):88–91. doi: 10.1128/aem.53.1.88-91.1987

Lysostaphin lysis procedure for detection of Staphylococcus aureus by the firefly bioluminescent ATP method.

E U Tuncan, S E Martin
PMCID: PMC203608  PMID: 3827251

Abstract

The objective of this study was to examine the use of lysostaphin as an ATP-extracting agent for the estimation of Staphylococcus aureus cell number by a rapid bioluminescent ATP method. The results of the study showed that lysostaphin (22 U/ml) was able to lyse most of the S. aureus cells (greater than 99.9%) at room temperature in 1 min; ATP of S. aureus cells extracted by the lysostaphin lysis procedure was stable for 24 h in the presence of EDTA; there was a linear relationship between the ATP content and the number of S. aureus cells (ranging from 10(4) to 10(6) CFU/ml); and the lysis of S. aureus cells by lysostaphin allowed estimation of the number of S. aureus cells in mixed cultures and in meat samples.

Full text

PDF
88

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWDER H. P., ZYGMUNT W. A., YOUNG J. R., TAVORMINA P. A. LYSOSTAPHIN: ENZYMATIC MODE OF ACTION. Biochem Biophys Res Commun. 1965 Apr 23;19:383–389. doi: 10.1016/0006-291x(65)90473-0. [DOI] [PubMed] [Google Scholar]
  2. Heddaeus H., Heczko P. B., Pulverer G. Evaluation of the lysostaphin-susceptibility test for the classification of staphylococci. J Med Microbiol. 1979 Feb;12(1):9–15. doi: 10.1099/00222615-12-1-9. [DOI] [PubMed] [Google Scholar]
  3. Iversen O. J., Grov A. Studies on lysostaphin. Separation and characterization of three enzymes. Eur J Biochem. 1973 Oct 5;38(2):293–300. doi: 10.1111/j.1432-1033.1973.tb03061.x. [DOI] [PubMed] [Google Scholar]
  4. McElroy W. D., Seliger H. H., White E. H. Mechanism of bioluminescence, chemiluminescence and enzyme function in the oxidation of firefly luciferin. Photochem Photobiol. 1969 Sep;10(3):153–170. doi: 10.1111/j.1751-1097.1969.tb05676.x. [DOI] [PubMed] [Google Scholar]
  5. SCHINDLER C. A., SCHUHARDT V. T. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proc Natl Acad Sci U S A. 1964 Mar;51:414–421. doi: 10.1073/pnas.51.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Severance P. J., Kauffman C. A., Sheagren J. N. Rapid identification of Staphylococcus aureus by using lysostaphin sensitivity. J Clin Microbiol. 1980 Jun;11(6):724–727. doi: 10.1128/jcm.11.6.724-727.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sharpe A. N., Woodrow M. N., Jackson A. K. Adenosinetriphosphate (ATP) levels in foods contaminated by bacteria. J Appl Bacteriol. 1970 Dec;33(4):758–767. doi: 10.1111/j.1365-2672.1970.tb02260.x. [DOI] [PubMed] [Google Scholar]
  8. Stannard C. J., Wood J. M. The rapid estimation of microbial contamination of raw meat by measurement of adenosine triphosphate (ATP). J Appl Bacteriol. 1983 Dec;55(3):429–438. doi: 10.1111/j.1365-2672.1983.tb01682.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES