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. 1987 Feb;53(2):320–324. doi: 10.1128/aem.53.2.320-324.1987

Selection of Streptococcus lactis Mutants Defective in Malolactic Fermentation

Pierre P Renault 1,*, Henri Heslot 1
PMCID: PMC203659  PMID: 16347282

Abstract

An enrichment medium and a new sensitive medium were developed to detect malolactic variants in different strains of lactic bacteria. Factors such as the concentration of glucose and l-malate, pH level, and the type of indicator dye used are discussed with regard to the kinetics of malic acid conversion to lactic acid. Use of these media allowed a rapid and easier screening of mutagenized streptococcal cells unable to ferment l-malate. A collection of malolactic-negative mutants of Streptococcus lactis induced by UV, nitrosoguanidine, or transposonal mutagenesis were characterized. The results showed that several mutants were apparently defective in the structural gene of malolactic enzyme, whereas others contained mutations which may either inactivate a putative permease or affect a regulatory sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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