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. 1987 Apr;53(4):665–671. doi: 10.1128/aem.53.4.665-671.1987

Mobilization of plasmid pHSV106 from Escherichia coli HB101 in a laboratory-scale waste treatment facility.

P Mancini, S Fertels, D Nave, M A Gealt
PMCID: PMC203733  PMID: 3555335

Abstract

The mobilization of plasmid pHSV106 from Escherichia coli HB101, in a laboratory model waste treatment facility, by both laboratory and indigenous wastewater strains of E. coli was monitored by transfer of antibiotic resistance characteristics and by detection of pHSV106 DNA sequences in recipient cells. The mobilization was demonstrated to occur under several different treatment conditions, such as different media composition, microbial concentrations, and waste flow rates. The herpes simplex virus thymidine kinase gene was used as a hybridization marker to confirm the occurrence of the transfer. The use of the HB101 (recA mutant) host cell implies that recA functions are unnecessary for the gene transfer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alcaide E., Garay E. R-plasmid transfer in Salmonella spp. isolated from wastewater and sewage-contaminated surface waters. Appl Environ Microbiol. 1984 Aug;48(2):435–438. doi: 10.1128/aem.48.2.435-438.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Finnegan J., Sherratt D. Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer. Mol Gen Genet. 1982;185(2):344–351. doi: 10.1007/BF00330810. [DOI] [PubMed] [Google Scholar]
  3. Gealt M. A., Chai M. D., Alpert K. B., Boyer J. C. Transfer of plasmids pBR322 and pBR325 in wastewater from laboratory strains of Escherichia coli to bacteria indigenous to the waste disposal system. Appl Environ Microbiol. 1985 Apr;49(4):836–841. doi: 10.1128/aem.49.4.836-841.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mach P. A., Grimes D. J. R-plasmid transfer in a wastewater treatment plant. Appl Environ Microbiol. 1982 Dec;44(6):1395–1403. doi: 10.1128/aem.44.6.1395-1403.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McKnight S. L., Gavis E. R. Expression of the herpes thymidine kinase gene in Xenopus laevis oocytes: an assay for the study of deletion mutants constructed in vitro. Nucleic Acids Res. 1980 Dec 20;8(24):5931–5948. doi: 10.1093/nar/8.24.5931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McKnight S. L. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res. 1980 Dec 20;8(24):5949–5964. doi: 10.1093/nar/8.24.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McPherson P., Gealt M. A. Isolation of indigenous wastewater bacterial strains capable of mobilizing plasmid pBR325. Appl Environ Microbiol. 1986 May;51(5):904–909. doi: 10.1128/aem.51.5.904-909.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stotzky G., Babich H. Fate of genetically-engineered microbes in natural environments. Recomb DNA Tech Bull. 1984 Dec;7(4):163–188. [PubMed] [Google Scholar]
  9. Taylor D. P., Cohen S. N., Clark W. G., Marrs B. L. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol. 1983 May;154(2):580–590. doi: 10.1128/jb.154.2.580-590.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Van Haute E., Joos H., Maes M., Warren G., Van Montagu M., Schell J. Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J. 1983;2(3):411–417. doi: 10.1002/j.1460-2075.1983.tb01438.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wagner M. J., Sharp J. A., Summers W. C. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441–1445. doi: 10.1073/pnas.78.3.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wolk C. P., Vonshak A., Kehoe P., Elhai J. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1561–1565. doi: 10.1073/pnas.81.5.1561. [DOI] [PMC free article] [PubMed] [Google Scholar]

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