Abstract
Little is known about the ability of wetlands to remove disease-causing viruses from municipal wastewater. In this study we examined the survival of several indicators of viral pollution (indigenous F-specific bacteriophages, seeded MS2 bacteriophage, and seeded human poliovirus type 1) applied in primary municipal wastewater to artificial wetland ecosystems. Only about 1% of the indigenous F-specific RNA bacteriophages survived flow through the vegetated wetland beds at a 5-cm-day-1 hydraulic application rate during the period from June through December 1985. The total number of indigenous F-specific bacteriophages (F-specific RNA and F-specific DNA phages) was also reduced by about 99% by wetland treatment, with the mean inflow concentration over the period of an entire year reduced from 3,129 to 33 PFU ml-1 in the outflow of a vegetated bed and to 174 PFU ml-1 in the outflow of an unvegetated bed. Such superior treatment by the vegetated bed demonstrates the significant role of higher aquatic plants in the removal process. Seeded MS2 bacteriophage and seeded poliovirus were removed more efficiently than were the indigenous bacteriophages, with less than 0.2% of MS2 and 0.1% of the poliovirus surviving flow at the same hydraulic application rate. The decay rate (k) of MS2 in a stagnant wetlands (k = 0.012 to 0.028 h-1) was lower than that for flowing systems (k = 0.44 to 0.052 h-1), reflecting the enhanced capacity for filtration or adsorption of viruses by the root-substrate complex (and associated biofilm).(ABSTRACT TRUNCATED AT 250 WORDS)
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- Ackermann H. W., Audurier A., Berthiaume L., Jones L. A., Mayo J. A., Vidaver A. K. Guidelines for bacteriophage characterization. Adv Virus Res. 1978;23:1–24. doi: 10.1016/s0065-3527(08)60096-2. [DOI] [PubMed] [Google Scholar]
- Cramer W. N., Kawata K., Krusé C. W. Chlorination and iodination of poliovirus and f2. J Water Pollut Control Fed. 1976 Jan;48(1):61–76. [PubMed] [Google Scholar]
- Grabow W. O., Middendorff I. G., Basson N. C. Role of lime treatment in the removal of bacteria, enteric viruses, and coliphages in a wastewater reclamation plant. Appl Environ Microbiol. 1978 Apr;35(4):663–669. doi: 10.1128/aem.35.4.663-669.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Havelaar A. H., Hogeboom W. M. A method for the enumeration of male-specific bacteriophages in sewage. J Appl Bacteriol. 1984 Jun;56(3):439–447. doi: 10.1111/j.1365-2672.1984.tb01372.x. [DOI] [PubMed] [Google Scholar]
- LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McConnell L. K., Sims R. C., Barnett B. B. Reovirus removal and inactivation by slow-rate sand filtration. Appl Environ Microbiol. 1984 Oct;48(4):818–825. doi: 10.1128/aem.48.4.818-825.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stetler R. E. Coliphages as indicators of enteroviruses. Appl Environ Microbiol. 1984 Sep;48(3):668–670. doi: 10.1128/aem.48.3.668-670.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward R. L., Ashley C. S. Inactivation of poliovirus in digested sludge. Appl Environ Microbiol. 1976 Jun;31(6):921–930. doi: 10.1128/aem.31.6.921-930.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wellings F. M., Lewis A. L., Mountain C. W., Pierce L. V. Demonstration of virus in groundwater after effluent discharge onto soil. Appl Microbiol. 1975 Jun;29(6):751–757. doi: 10.1128/am.29.6.751-757.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wentsel R. S., O'Neill P. E., Kitchens J. F. Evaluation of coliphage detection as a rapid indicator of water quality. Appl Environ Microbiol. 1982 Feb;43(2):430–434. doi: 10.1128/aem.43.2.430-434.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yates M. V., Gerba C. P., Kelley L. M. Virus persistence in groundwater. Appl Environ Microbiol. 1985 Apr;49(4):778–781. doi: 10.1128/aem.49.4.778-781.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]