Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Apr;53(4):816–822. doi: 10.1128/aem.53.4.816-822.1987

Restriction Fragment Length Polymorphisms in the Mushrooms Agaricus brunnescens and Agaricus bitorquis

Alan J Castle 1,*, Paul A Horgen 1, James B Anderson 1
PMCID: PMC203762  PMID: 2907275

Abstract

Two Agaricus species, A. brunnescens (a commercial mushroom) and A. bitorquis (a wild, edible species), were examined for restriction fragment length polymorphisms. EcoRI-digested nuclear DNA from isolates of both species were cloned in plasmid vector pUC18. Ten random recombinant clones were used in Southern DNA-DNA hybridizations to probe EcoRI-digested DNA from 11 A. brunnescens isolates (7 commercial, 2 wild type, and 2 homokaryotic) and 7 A. bitorquis isolates. Most cloned fragments were polymorphic in both species. There were fewer different genotypes than expected, however, in the sample of commercial A. brunnescens strains. DNA from homokaryotic strains showed fewer bands in most hybridizations than DNA from heterokaryotic strains. All A. bitorquis isolates could be distinguished from each other as well as from every A. brunnescens strain. Putative homokaryons were detected by the loss of polymorphic bands among protoplast regenerates from one commercial strain and two strains collected in the wild.

Full text

PDF
816

Images in this article

819Image
on p.819
820Image
on p.820
820Image
on p.820

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baas F., Bikker H., van Ommen G. J., de Vijlder J. J. Unusual scarcity of restriction site polymorphism in the human thyroglobulin gene. A linkage study suggesting autosomal dominance of a defective thyroglobulin allele. Hum Genet. 1984;67(3):301–305. doi: 10.1007/BF00291357. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., White R. L., Skolnick M., Davis R. W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed] [Google Scholar]
  3. EVANS H. J. Nuclear behaviour in the cultivated mushroom. Chromosoma. 1959;10(2):115–135. doi: 10.1007/BF00396566. [DOI] [PubMed] [Google Scholar]
  4. Fredrickson B. E., Yuan H. A., Miller H. Burst fractures of the fifth lumbar vertebra. A report of four cases. J Bone Joint Surg Am. 1982 Sep;64(7):1088–1094. [PubMed] [Google Scholar]
  5. Hudspeth M. E., Shumard D. S., Tatti K. M., Grossman L. I. Rapid purification of yeast mitochondrial DNA in high yield. Biochim Biophys Acta. 1980 Dec 11;610(2):221–228. doi: 10.1016/0005-2787(80)90003-9. [DOI] [PubMed] [Google Scholar]
  6. Jackson P. R., Wohlhieter J. A., Jackson J. E., Sayles P., Diggs C. L., Hockmeyer W. T. Restriction endonuclease analysis of Leishmania kinetoplast DNA characterizes parasites responsible for visceral and cutaneous disease. Am J Trop Med Hyg. 1984 Sep;33(5):808–819. doi: 10.4269/ajtmh.1984.33.808. [DOI] [PubMed] [Google Scholar]
  7. Murray M. G., Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. doi: 10.1093/nar/8.19.4321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  9. Wyman A. R., White R. A highly polymorphic locus in human DNA. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754–6758. doi: 10.1073/pnas.77.11.6754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Zolan M. E., Pukkila P. J. Inheritance of DNA methylation in Coprinus cinereus. Mol Cell Biol. 1986 Jan;6(1):195–200. doi: 10.1128/mcb.6.1.195. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES