Abstract
Direct plating, selective enrichment, and cold enrichment followed by secondary selective enrichment procedures were compared for detecting and enumerating Listeria monocytogenes in chopped cabbage stored at 5 degrees C for up to 64 days. Addition of Fe3+ to solid media enhanced detection of the organism. Cold enrichment (5 degrees C) in nutrient broth and brain heart infusion broth followed by secondary enrichment (48 h, 30 degrees C) in Trypticase soy-yeast extract-antibiotic broth and thiocyanate-nalidixic acid broth and plating on selective agar media (Doyle and Schoeni selective enrichment agar [minus acriflavin hydrochloride, supplemented with 5 micrograms of Fe3+/ml] and McBride Listeria agar) resulted in the detection of highest populations.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beuchat L. R., Brackett R. E., Hao D. Y., Conner D. E. Growth and thermal inactivation of Listeria monocytogenes in cabbage and cabbage juice. Can J Microbiol. 1986 Oct;32(10):791–795. doi: 10.1139/m86-145. [DOI] [PubMed] [Google Scholar]
- Conner D. E., Brackett R. E., Beuchat L. R. Effect of temperature, sodium chloride, and pH on growth of Listeria monocytogenes in cabbage juice. Appl Environ Microbiol. 1986 Jul;52(1):59–63. doi: 10.1128/aem.52.1.59-63.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cowart R. E., Foster B. G. Differential effects of iron on the growth of Listeria monocytogenes: minimum requirements and mechanism of acquisition. J Infect Dis. 1985 Apr;151(4):721–730. doi: 10.1093/infdis/151.4.721. [DOI] [PubMed] [Google Scholar]
- Doyle M. P., Schoeni J. L. Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens. Appl Environ Microbiol. 1986 May;51(5):1127–1129. doi: 10.1128/aem.51.5.1127-1129.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khan M. A., Seaman A., Woodbine M. Differential media in the isolation of Listeria monocytogenes. Zentralbl Bakteriol Orig A. 1973 Aug;224(3):362–375. [PubMed] [Google Scholar]
- Martin R. S., Sumarah R. K., MacDonald M. A. A synthetic based medium for the isolation of Listeria monocytogenes. Clin Invest Med. 1984;7(4):233–237. [PubMed] [Google Scholar]
- Schlech W. F., 3rd, Lavigne P. M., Bortolussi R. A., Allen A. C., Haldane E. V., Wort A. J., Hightower A. W., Johnson S. E., King S. H., Nicholls E. S. Epidemic listeriosis--evidence for transmission by food. N Engl J Med. 1983 Jan 27;308(4):203–206. doi: 10.1056/NEJM198301273080407. [DOI] [PubMed] [Google Scholar]
- Watkins J., Sleath K. P. Isolation and enumeration of Listeria monocytogenes from Sewage, Sewage Sludge and River Water. J Appl Bacteriol. 1981 Feb;50(1):1–9. doi: 10.1111/j.1365-2672.1981.tb00865.x. [DOI] [PubMed] [Google Scholar]
- Welshimer H. J. Isolation of Listeria monocytogenes from vegetation. J Bacteriol. 1968 Feb;95(2):300–303. doi: 10.1128/jb.95.2.300-303.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]