Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 May;53(5):969–973. doi: 10.1128/aem.53.5.969-973.1987

Nucleotide and deduced amino acid sequences of the Staphylococcus aureus phospho-beta-galactosidase gene.

F Breidt Jr, G C Stewart
PMCID: PMC203796  PMID: 3111370

Abstract

We sequenced the Staphylococcus aureus phospho-beta-galactosidase gene. The protein product of this gene consisted of 470 amino acids, giving a molecular weight of 54,557. This gene appears to be transcribed as the terminal sequence on a polycistronic message.

Full text

PDF
969

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breidt F., Jr, Stewart G. C. Cloning and expression of the phospho-beta-galactosidase gene of Staphylococcus aureus in Escherichia coli. J Bacteriol. 1986 Jun;166(3):1061–1066. doi: 10.1128/jb.166.3.1061-1066.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Green C. J., Stewart G. C., Hollis M. A., Vold B. S., Bott K. F. Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB. Gene. 1985;37(1-3):261–266. doi: 10.1016/0378-1119(85)90281-1. [DOI] [PubMed] [Google Scholar]
  4. Harlander S. K., McKay L. L., Schachtele C. F. Molecular cloning of the lactose-metabolizing genes from Streptococcus lactis. Appl Environ Microbiol. 1984 Aug;48(2):347–351. doi: 10.1128/aem.48.2.347-351.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hengstenberg W., Penberthy W. K., Hill K. L., Morse M. L. Metabolism of lactose by Staphylococcus aureus. J Bacteriol. 1968 Dec;96(6):2187–2188. doi: 10.1128/jb.96.6.2187-2188.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hengstenberg W., Penberthy W. K., Morse M. L. Purification of the staphylococcal 6-phospho-beta-D-- galactosidase. Eur J Biochem. 1970 May 1;14(1):27–32. doi: 10.1111/j.1432-1033.1970.tb00256.x. [DOI] [PubMed] [Google Scholar]
  7. Inamine J. M., Lee L. N., LeBlanc D. J. Molecular and genetic characterization of lactose-metabolic genes of Streptococcus cremoris. J Bacteriol. 1986 Sep;167(3):855–862. doi: 10.1128/jb.167.3.855-862.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee C. Y., Iandolo J. J. Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene. J Bacteriol. 1986 May;166(2):385–391. doi: 10.1128/jb.166.2.385-391.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee L. J., Hansen J. B., Jagusztyn-Krynicka E. K., Chassy B. M. Cloning and expression of the beta-D-phosphogalactoside galactohydrolase gene of Lactobacillus casei in Escherichia coli K-12. J Bacteriol. 1982 Dec;152(3):1138–1146. doi: 10.1128/jb.152.3.1138-1146.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maeda S., Gasson M. J. Cloning, expression and location of the Streptococcus lactis gene for phospho-beta-D-galactosidase. J Gen Microbiol. 1986 Feb;132(2):331–340. doi: 10.1099/00221287-132-2-331. [DOI] [PubMed] [Google Scholar]
  11. McLaughlin J. R., Murray C. L., Rabinowitz J. C. Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. J Biol Chem. 1981 Nov 10;256(21):11283–11291. [PubMed] [Google Scholar]
  12. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  13. Mongkolsuk S., Duvall E. J., Lovett P. S. Transcription termination signal for the cat-86 indicator gene in a Bacillus subtilis promoter-cloning plasmid. Gene. 1985;37(1-3):83–90. doi: 10.1016/0378-1119(85)90260-4. [DOI] [PubMed] [Google Scholar]
  14. Moran C. P., Jr, Lang N., LeGrice S. F., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982;186(3):339–346. doi: 10.1007/BF00729452. [DOI] [PubMed] [Google Scholar]
  15. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shortle D. A genetic system for analysis of staphylococcal nuclease. Gene. 1983 May-Jun;22(2-3):181–189. doi: 10.1016/0378-1119(83)90102-6. [DOI] [PubMed] [Google Scholar]
  18. Snook R. J., McKay L. L. Conjugal Transfer of Lactose-Fermenting Ability Among Streptococcus cremoris and Streptococcus lactis Strains. Appl Environ Microbiol. 1981 Nov;42(5):904–911. doi: 10.1128/aem.42.5.904-911.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tinoco I., Jr, Borer P. N., Dengler B., Levin M. D., Uhlenbeck O. C., Crothers D. M., Bralla J. Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973 Nov 14;246(150):40–41. doi: 10.1038/newbio246040a0. [DOI] [PubMed] [Google Scholar]
  20. Uhlén M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M. Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. J Biol Chem. 1984 Feb 10;259(3):1695–1702. [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES