Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 May;53(5):1034–1041. doi: 10.1128/aem.53.5.1034-1041.1987

Isolation of a Cellodextrinase from Bacteroides succinogenes

Li Huang 1, Cecil W Forsberg 1,*
PMCID: PMC203805  PMID: 16347334

Abstract

An enzyme which released the cellobiose group from p-nitrophenyl cellobioside was isolated from the periplasmic space of Bacteroides succinogenes grown on Avicel crystalline cellulose in a continuous cultivation system and separated from endoglucanases by column chromatography. The molecular weight of the enzyme was approximately 40,000, as estimated by gel filtration. The enzyme has an isoelectric point of 4.9. The enzyme exhibited low hydrolytic activity on acid-swollen cellulose and practically no activity on carboxymethyl cellulose, Avicel cellulose, and cellobiose, but it hydrolyzed p-nitrophenyl lactoside and released cellobiose from cellotriose and from higher cello-oligosaccharides. These data demonstrate that the enzyme is a cellodextrinase with an exotype of function.

Full text

PDF
1034

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AYERS W. A. Phosphorylation of cellobiose and glucose by Ruminococcus flavefaciens. J Bacteriol. 1958 Nov;76(5):515–517. doi: 10.1128/jb.76.5.515-517.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beacham I. R. Periplasmic enzymes in gram-negative bacteria. Int J Biochem. 1979;10(11):877–883. doi: 10.1016/0020-711x(79)90117-4. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Deshpande M. V., Eriksson K. E., Pettersson L. G. An assay for selective determination of exo-1,4,-beta-glucanases in a mixture of cellulolytic enzymes. Anal Biochem. 1984 May 1;138(2):481–487. doi: 10.1016/0003-2697(84)90843-1. [DOI] [PubMed] [Google Scholar]
  5. Forsberg C. W., Beveridge T. J., Hellstrom A. Cellulase and Xylanase Release from Bacteroides succinogenes and Its Importance in the Rumen Environment. Appl Environ Microbiol. 1981 Nov;42(5):886–896. doi: 10.1128/aem.42.5.886-896.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedberg I. Localization of phosphoglucose isomerase in Escherichia coli and its relation to the induction of the hexose phosphate transport system. J Bacteriol. 1972 Dec;112(3):1201–1205. doi: 10.1128/jb.112.3.1201-1205.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilkes N. R., Langsford M. L., Kilburn D. G., Miller R. C., Jr, Warren R. A. Mode of action and substrate specificities of cellulases from cloned bacterial genes. J Biol Chem. 1984 Aug 25;259(16):10455–10459. [PubMed] [Google Scholar]
  8. Groleau D., Forsberg C. W. Cellulolytic activity of the rumen bacterium Bacteroides succinogenes. Can J Microbiol. 1981 May;27(5):517–530. doi: 10.1139/m81-077. [DOI] [PubMed] [Google Scholar]
  9. Groleau D., Forsberg C. W. Partial characterization of the extracellular carboxymethylcellulase activity produced by the rumen bacterium Bacteroides succinogenes. Can J Microbiol. 1983 May;29(5):504–517. doi: 10.1139/m83-080. [DOI] [PubMed] [Google Scholar]
  10. Gum E. K., Jr, Brown R. D., Jr Comparison of four purified extracellular 1,4-beta-D-glucan cellobiohydrolase enzymes from Trichoderma viride. Biochim Biophys Acta. 1977 May 27;492(1):225–231. doi: 10.1016/0005-2795(77)90229-x. [DOI] [PubMed] [Google Scholar]
  11. HALLIWELL G., BRYANT M. P. THE CELLULOLYTIC ACTIVITY OF PURE STRAINS OF BACTERIA FROM THE RUMEN OF CATTLE. J Gen Microbiol. 1963 Sep;32:441–448. doi: 10.1099/00221287-32-3-441. [DOI] [PubMed] [Google Scholar]
  12. Halliwell G., Griffin M. The nature and mode of action of the cellulolytic component C1 of Trichoderma koningii on native cellulose. Biochem J. 1973 Dec;135(4):587–594. doi: 10.1042/bj1350587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lee S. F., Forsberg C. W., Gibbins L. N. Xylanolytic Activity of Clostridium acetobutylicum. Appl Environ Microbiol. 1985 Oct;50(4):1068–1076. doi: 10.1128/aem.50.4.1068-1076.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MALAMY M., HORECKER B. L. The localization of alkaline phosphatase in E. coli K12. Biochem Biophys Res Commun. 1961 Jun 2;5:104–108. doi: 10.1016/0006-291x(61)90020-1. [DOI] [PubMed] [Google Scholar]
  15. MacKenzie C. R., Bilous D., Johnson K. G. Purification and characterization of an exoglucanase from Streptomyces flavogriseus. Can J Microbiol. 1984 Sep;30(9):1171–1178. doi: 10.1139/m84-183. [DOI] [PubMed] [Google Scholar]
  16. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  17. Ng T. K., Zeikus J. G. Comparison of Extracellular Cellulase Activities of Clostridium thermocellum LQRI and Trichoderma reesei QM9414. Appl Environ Microbiol. 1981 Aug;42(2):231–240. doi: 10.1128/aem.42.2.231-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ohmiya K., Shimizu M., Taya M., Shimizu S. Purification and properties of cellobiosidase from Ruminococcus albus. J Bacteriol. 1982 Apr;150(1):407–409. doi: 10.1128/jb.150.1.407-409.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ohmiya K., Shirai M., Kurachi Y., Shimizu S. Isolation and properties of beta-glucosidase from Ruminococcus albus. J Bacteriol. 1985 Jan;161(1):432–434. doi: 10.1128/jb.161.1.432-434.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCOTT H. W., DEHORITY B. A. VITAMIN REQUIREMENTS OF SEVERAL CELLULOLYTIC RUMEN BACTERIA. J Bacteriol. 1965 May;89:1169–1175. doi: 10.1128/jb.89.5.1169-1175.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor K. A., Crosby B., McGavin M., Forsberg C. W., Thomas D. Y. Characteristics of the endoglucanase encoded by a cel gene from Bacteroides succinogenes expressed in Escherichia coli. Appl Environ Microbiol. 1987 Jan;53(1):41–46. doi: 10.1128/aem.53.1.41-46.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wood T. M., McCrae S. I. The purification and properties of the C 1 component of Trichoderma koningii cellulase. Biochem J. 1972 Aug;128(5):1183–1192. doi: 10.1042/bj1281183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wood T. M. Properties of cellulolytic enzyme systems. Biochem Soc Trans. 1985 Apr;13(2):407–410. doi: 10.1042/bst0130407. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES