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. 1987 May;53(5):1069–1072. doi: 10.1128/aem.53.5.1069-1072.1987

Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.

M Yamamoto, J M Jones, E Senghas, C Gawron-Burke, D B Clewell
PMCID: PMC203811  PMID: 3038014

Abstract

A method has been developed for the introduction of Tn5 into Escherichia coli plasmid chimeras containing Streptococcus faecalis DNA. Tn5 could be introduced via a lambda::Tn5 delivery vehicle. The system proved to be particularly efficient and facilitated insertions at numerous sites on DNA containing the 16-kilobase conjugative transposon Tn916. It was possible to introduce some of the resulting Tn916::Tn5 derivatives back into S. faecalis by using a recently developed protoplast transformation procedure. A presumed zygotic induction resulted in insertion of the Tn916 derivatives at multiple sites in the S. faecalis chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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