Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 May;53(5):1171–1174. doi: 10.1128/aem.53.5.1171-1174.1987

Restriction enzyme analysis of lactose and bacteriocin plasmids from Streptococcus lactis subsp. diacetylactis WM4 and cloning of BclI fragments coding for bacteriocin production.

K S Harmon, L L McKay
PMCID: PMC203827  PMID: 3038015

Abstract

The 131.1-kilobase (kb) bacteriocin production (Bac) plasmid pNP2 and the 63.6-kb lactose metabolism (Lac) plasmid pCS26, from Streptococcus lactis subsp. diacetylactis WM4, as well as pWN8, a 116.7-kb recombinant plasmid from a Lac+ transconjugant, were analyzed with restriction enzymes to determine the origin of pWN8. Plasmid pWN8 conferred a Lac+ Bac- phenotype, contained DNA derived from pCS26 and pNP2, and, like pNP2, exhibited self-transmissibility (Tra+). In cloning attempts, Bac+ transformant S. lactis KSH1 was isolated. The recombinant plasmid, pKSH1, contained three BclI fragments from pNP2. Bac- transformants which individually contained each of the three fragments were also identified. Comparison of restriction maps of pKSH1 and pNP2 revealed an 18.4-kb region common to both plasmids, involving two of the three BclI fragments. S. lactis KSH1 also exhibited greater inhibitory activity against the indicator strain S. diacetylactis 18-16 than did a strain containing the 131.1-kb Bac plasmid.

Full text

PDF
1171

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. G., McKay L. L. Genetic and physical characterization of recombinant plasmids associated with cell aggregation and high-frequency conjugal transfer in Streptococcus lactis ML3. J Bacteriol. 1984 Jun;158(3):954–962. doi: 10.1128/jb.158.3.954-962.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson D. G., McKay L. L. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. doi: 10.1128/aem.46.3.549-552.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Behnke D., Gilmore M. S., Ferretti J. J. Plasmid pGB301, a new multiple resistance streptococcal cloning vehicle and its use in cloning of a gentamicin/kanamycin resistance determinant. Mol Gen Genet. 1981;182(3):414–421. doi: 10.1007/BF00293929. [DOI] [PubMed] [Google Scholar]
  4. Davey G. P. Plasmid associated with diplococcin production in Streptococcus. Appl Environ Microbiol. 1984 Oct;48(4):895–896. doi: 10.1128/aem.48.4.895-896.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gasson M. J., Davies F. L. High-frequency conjugation associated with Streptococcus lactis donor cell aggregation. J Bacteriol. 1980 Sep;143(3):1260–1264. doi: 10.1128/jb.143.3.1260-1264.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kondo J. K., McKay L. L. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA. Appl Environ Microbiol. 1982 May;43(5):1213–1215. doi: 10.1128/aem.43.5.1213-1215.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kékessy D. A., Piguet J. D. New method for detecting bacteriocin production. Appl Microbiol. 1970 Aug;20(2):282–283. doi: 10.1128/am.20.2.282-283.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McHugh G. L., Swartz M. N. Elimination of plasmids from several bacterial species by novobiocin. Antimicrob Agents Chemother. 1977 Sep;12(3):423–426. doi: 10.1128/aac.12.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKay L. L., Baldwin K. A., Walsh P. M. Conjugal transfer of genetic information in group N streptococci. Appl Environ Microbiol. 1980 Jul;40(1):84–89. doi: 10.1128/aem.40.1.84-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McKay L. L., Cords B. R., Baldwin K. A. Transduction of lactose metabolism in Streptococcus lactis C2. J Bacteriol. 1973 Sep;115(3):810–815. doi: 10.1128/jb.115.3.810-815.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Neve H., Geis A., Teuber M. Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci. J Bacteriol. 1984 Mar;157(3):833–838. doi: 10.1128/jb.157.3.833-838.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scherwitz K. M., Baldwin K. A., McKay L. L. Plasmid linkage of a bacteriocin-like substance in Streptococcus lactis subsp. diacetylactis strain WM4: transferability to Streptococcus lactis. Appl Environ Microbiol. 1983 May;45(5):1506–1512. doi: 10.1128/aem.45.5.1506-1512.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Steele J. L., McKay L. L. Partial characterization of the genetic basis for sucrose metabolism and nisin production in Streptococcus lactis. Appl Environ Microbiol. 1986 Jan;51(1):57–64. doi: 10.1128/aem.51.1.57-64.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walsh P. M., McKay L. L. Recombinant plasmid associated cell aggregation and high-frequency conjugation of Streptococcus lactis ML3. J Bacteriol. 1981 Jun;146(3):937–944. doi: 10.1128/jb.146.3.937-944.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES