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. 1987 May;53(5):1192–1195. doi: 10.1128/aem.53.5.1192-1195.1987

An A-ELISA to detect hepatitis A virus in estuarine samples.

A M Nasser, T G Metcalf
PMCID: PMC203834  PMID: 3038016

Abstract

An amplified enzyme-linked immunosorbent assay (A-ELISA) for detecting and quantifying hepatitis A virus in estuarine water samples is described. The test was five times more sensitive than a standard ELISA and at least two times more sensitive than radioimmunoassay. Test sensitivity was unaffected by the procedures used to concentrate the virus in estuarine samples or by the presence of humic and tannic acids in test samples. Nonspecific reactions were not encountered with a number of enteroviruses or with a rotavirus. A high sensitivity and specificity combined with speed, low cost, and freedom from radiolabels made the A-ELISA useful for detecting hepatitis A virus in environmental samples. The virus was detected in 3 of 20 estuarine water samples examined by A-ELISA.

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Selected References

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  1. Adler-Storthz K., Kendall C., Kennedy R. C., Henkel R. D., Dreesman G. R. Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens. J Clin Microbiol. 1983 Dec;18(6):1329–1334. doi: 10.1128/jcm.18.6.1329-1334.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulepis A. G., Veale M. F., MacGregor A., Kornitschuk M., Gust I. D. Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies. J Clin Microbiol. 1985 Jul;22(1):119–124. doi: 10.1128/jcm.22.1.119-124.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Emini E. A., Jameson B. A., Lewis A. J., Larsen G. R., Wimmer E. Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies. J Virol. 1982 Sep;43(3):997–1005. doi: 10.1128/jvi.43.3.997-1005.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feinstone S. M., Kapikian A. Z., Purceli R. H. Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness. Science. 1973 Dec 7;182(4116):1026–1028. doi: 10.1126/science.182.4116.1026. [DOI] [PubMed] [Google Scholar]
  5. Gary G. W., Jr, Kaplan J. E., Stine S. E., Anderson L. J. Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay. J Clin Microbiol. 1985 Aug;22(2):274–278. doi: 10.1128/jcm.22.2.274-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guesdon J. L., Ternynck T., Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979 Aug;27(8):1131–1139. doi: 10.1177/27.8.90074. [DOI] [PubMed] [Google Scholar]
  7. Hall W. T., Bradley D. W., Madden D. L., Zimmerman D. H., Brandt D. E. Comparison of sensitivity of radioimmunoassay and immune electron microscopy for detecting hepatitis A antigen in fecal extracts. Proc Soc Exp Biol Med. 1977 Jun;155(2):193–198. doi: 10.3181/00379727-155-39772. [DOI] [PubMed] [Google Scholar]
  8. Harris C. C., Yolken R. H., Krokan H., Hsu I. C. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5336–5339. doi: 10.1073/pnas.76.10.5336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollinger F. B., Bradley D. W., Maynard J. E., Dreesman G. R., Melnick J. L. Detection of hepatitis A viral antigen by radioimmunoassay. J Immunol. 1975 Nov;115(5):1464–1466. [PubMed] [Google Scholar]
  10. Jiang X., Estes M. K., Metcalf T. G., Melnick J. L. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes. Appl Environ Microbiol. 1986 Oct;52(4):711–717. doi: 10.1128/aem.52.4.711-717.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katzenelson E., Fattal B., Hostovesky T. Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water. Appl Environ Microbiol. 1976 Oct;32(4):638–639. doi: 10.1128/aem.32.4.638-639.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Latham R. H., Schable C. A. Foodborne hepatitis A at a family reunion use of IgM-specific hepatitis a serologic testing. Am J Epidemiol. 1982 May;115(5):640–645. doi: 10.1093/oxfordjournals.aje.a113346. [DOI] [PubMed] [Google Scholar]
  13. Salacinski P. R., McLean C., Sykes J. E., Clement-Jones V. V., Lowry P. J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981 Oct;117(1):136–146. doi: 10.1016/0003-2697(81)90703-x. [DOI] [PubMed] [Google Scholar]
  14. Simmonds R. S., Szücs G., Metcalf T. G., Melnick J. L. Persistently infected cultures as a source of hepatitis A virus. Appl Environ Microbiol. 1985 Apr;49(4):749–755. doi: 10.1128/aem.49.4.749-755.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
  16. Yolken R. H., Stopa P. J. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus. J Clin Microbiol. 1979 Sep;10(3):317–321. doi: 10.1128/jcm.10.3.317-321.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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