Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Jun;53(6):1246–1250. doi: 10.1128/aem.53.6.1246-1250.1987

Improved membrane filtration media for enumeration of total coliforms and Escherichia coli from sewage and surface waters.

T A Freier, P A Hartman
PMCID: PMC203849  PMID: 3300547

Abstract

Two media were developed that allowed both a total coliform count and an Escherichia coli count to be determined on the same medium after 24 h of incubation at 35 degrees C. The new media were tested along with two standard media on 10 surface water and 7 sewage samples. The experimental media yielded equivalent or higher counts relative to the standard media and recovered more specifically the desired indicator groups as determined by colony identification.

Full text

PDF
1246

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burman N. P., Colbourne J. S. Techniques for the assessment of growth of micro-organisms on plumbing materials used in contact with potable water supplies. J Appl Bacteriol. 1977 Aug;43(1):137–144. doi: 10.1111/j.1365-2672.1977.tb00732.x. [DOI] [PubMed] [Google Scholar]
  2. Camper A. K., McFeters G. A. Chlorine injury and the enumeration of waterborne coliform bacteria. Appl Environ Microbiol. 1979 Mar;37(3):633–641. doi: 10.1128/aem.37.3.633-641.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caplenas N. R., Kanarek M. S. Thermotolerant non-fecal source Klebsiella pneumoniae: validity of the fecal coliform test in recreational waters. Am J Public Health. 1984 Nov;74(11):1273–1275. doi: 10.2105/ajph.74.11.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Domek M. J., LeChevallier M. W., Cameron S. C., McFeters G. A. Evidence for the role of copper in the injury process of coliform bacteria in drinking water. Appl Environ Microbiol. 1984 Aug;48(2):289–293. doi: 10.1128/aem.48.2.289-293.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dufour A. P., Strickland E. R., Cabelli V. J. Membrane filter method for enumerating Escherichia coli. Appl Environ Microbiol. 1981 May;41(5):1152–1158. doi: 10.1128/aem.41.5.1152-1158.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feng P. C., Hartman P. A. Fluorogenic assays for immediate confirmation of Escherichia coli. Appl Environ Microbiol. 1982 Jun;43(6):1320–1329. doi: 10.1128/aem.43.6.1320-1329.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOODWIN R. H., KAVANAGH F. Fluorescence of coumarin derivatives as a function of pH. Arch Biochem. 1950 Jun;27(1):152–173. [PubMed] [Google Scholar]
  8. Godsey J. H., Matteo M. R., Shen D., Tolman G., Gohlke J. R. Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles. J Clin Microbiol. 1981 Mar;13(3):483–490. doi: 10.1128/jcm.13.3.483-490.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hansen W., Yourassowsky E. Detection of beta-glucuronidase in lactose-fermenting members of the family Enterobacteriaceae and its presence in bacterial urine cultures. J Clin Microbiol. 1984 Dec;20(6):1177–1179. doi: 10.1128/jcm.20.6.1177-1179.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hufham J. B. Evaluating the membrane fecal coliform test by using Escherichia coli as the indicator organism. Appl Microbiol. 1974 Apr;27(4):771–776. doi: 10.1128/am.27.4.771-776.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kilian M., Bülow P. Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases. Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):245–251. doi: 10.1111/j.1699-0463.1976.tb01933.x. [DOI] [PubMed] [Google Scholar]
  12. Le Minor L., Buissière J., Novel G., Novel M. Relation entre le sérotype et l'activité beta-glucuronidasique chez les Salmonella. Ann Microbiol (Paris) 1978 Aug-Sep;129B(2):155–165. [PubMed] [Google Scholar]
  13. LeChevallier M. W., Cameron S. C., McFeters G. A. New medium for improved recovery of coliform bacteria from drinking water. Appl Environ Microbiol. 1983 Feb;45(2):484–492. doi: 10.1128/aem.45.2.484-492.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LeChevallier M. W., Jakanoski P. E., Camper A. K., McFeters G. A. Evaluation of m-T7 agar as a fecal coliform medium. Appl Environ Microbiol. 1984 Aug;48(2):371–375. doi: 10.1128/aem.48.2.371-375.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McFeters G. A., Cameron S. C., LeChevallier M. W. Influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. Appl Environ Microbiol. 1982 Jan;43(1):97–103. doi: 10.1128/aem.43.1.97-103.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McFeters G. A., Kippin J. S., LeChevallier M. W. Injured coliforms in drinking water. Appl Environ Microbiol. 1986 Jan;51(1):1–5. doi: 10.1128/aem.51.1.1-5.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Palumbo S. A., Maxino F., Williams A. C., Buchanan R. L., Thayer D. W. Starch-Ampicillin Agar for the Quantitative Detection of Aeromonas hydrophila. Appl Environ Microbiol. 1985 Oct;50(4):1027–1030. doi: 10.1128/aem.50.4.1027-1030.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Petzel J. P., Hartman P. A. Monensin-based medium for determination of total gram-negative bacteria and Escherichia coli. Appl Environ Microbiol. 1985 Apr;49(4):925–933. doi: 10.1128/aem.49.4.925-933.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rippey S. R., Cabelli V. J. Membrane filter procedure for enumeration of Aeromonas hydrophila in fresh waters. Appl Environ Microbiol. 1979 Jul;38(1):108–113. doi: 10.1128/aem.38.1.108-113.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Trepeta R. W., Edberg S. C. Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. J Clin Microbiol. 1984 Feb;19(2):172–174. doi: 10.1128/jcm.19.2.172-174.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wright R. C. A new selective and differential agar medium for Escherichia coli and coliform organisms. J Appl Bacteriol. 1984 Jun;56(3):381–388. doi: 10.1111/j.1365-2672.1984.tb01365.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES