Abstract
A medium was developed for the differential enumeration of homofermentative and heterofermentative lactic acid bacteria. Essential components of the medium included fructose (14 mM), KH2PO4 (18 mM), bromcresol green (as a pH indicator), and other nutrients to support growth. In agar medium, homofermentative colonies were blue to green, while heterofermentative colonies remained white. A total of 21 Lactobacillus, Pediococcus, Leuconostoc, and Streptococcus species were correctly classified with the medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Daeschel M. A., McFeeters R. F., Fleming H. P., Klaenhammer T. R., Sanozky R. B. Mutation and Selection of Lactobacillus plantarum Strains That Do Not Produce Carbon Dioxide from Malate. Appl Environ Microbiol. 1984 Feb;47(2):419–420. doi: 10.1128/aem.47.2.419-420.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson C. M., Cronin F. Medium for the selective enumeration of lactic acid bacteria from foods. Appl Microbiol. 1973 Sep;26(3):439–440. doi: 10.1128/am.26.3.439-440.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KHAIRAT O. A FERMENTATION TUBE AND MEDIUM FOR FASTIDIOUS ANAEROBES. Can J Microbiol. 1964 Aug;10:513–519. doi: 10.1139/m64-064. [DOI] [PubMed] [Google Scholar]
- ROGOSA M., MITCHELL J. A., WISEMAN R. F. A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J Bacteriol. 1951 Jul;62(1):132–133. doi: 10.1128/jb.62.1.132-133.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sims W. Use of fusidic acid for differentiating homofermentative from heterofermentative lactobacilli. J Dent Res. 1968 Jan-Feb;47(1):105–107. doi: 10.1177/00220345680470010101. [DOI] [PubMed] [Google Scholar]