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. 1987 Jun;53(6):1385–1386. doi: 10.1128/aem.53.6.1385-1386.1987

Construction of Streptococcus lactis subsp. lactis Strains with a Single Plasmid Associated with Mucoid Phenotype

Atte von Wright 1,*, Soile Tynkkynen 1
PMCID: PMC203875  PMID: 16347368

Abstract

Lactose-fermenting mucoid (Lac+ Muc+) variants of plasmid-free Streptococcus lactis subsp. lactis MG1614 were obtained by protoplast transformation with total plasmid DNA from Muc+S. lactis subsp. cremoris ARH87. By using plasmid DNA from these variants for further transformations followed by novobiocininduced plasmid curing, Lac− Muc+ MG1614 strains containing only a single 30-megadalton plasmid could be constructed. This plasmid, designated pVS5, appeared to be associated with the Muc+ phenotype.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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