Abstract
Enrichment cultures were used to determine the conditions promoting fastest methanogenic propionate degradation and growth by adapting the cultures to various physical and chemical conditions and measuring the specific growth rate. We found that the fastest growth of propionate oxidizers occurred at pH 6.8 to 8.5 and 32 to 45°C. Acetate-degrading populations showed narrower optima for fastest growth (pH 6.8 to 7.2 and 37 to 43°C). Enrichment cultures grew as well in minimal medium as in complex medium, although individual microbial populations appeared to require growth factors which could be met by cross-feeding.
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Selected References
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- Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boone D. R., Bryant M. P. Propionate-Degrading Bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from Methanogenic Ecosystems. Appl Environ Microbiol. 1980 Sep;40(3):626–632. doi: 10.1128/aem.40.3.626-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boone D. R., Menaia J. A., Boone J. E., Mah R. A. Effects of Hydrogen Pressure during Growth and Effects of Pregrowth with Hydrogen on Acetate Degradation by Methanosarcina Species. Appl Environ Microbiol. 1987 Jan;53(1):83–87. doi: 10.1128/aem.53.1.83-87.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boone D. R. Mixed-culture fermentor for simulating methanogenic digestors. Appl Environ Microbiol. 1984 Jul;48(1):122–126. doi: 10.1128/aem.48.1.122-126.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boone D. R. Terminal reactions in the anaerobic digestion of animal waste. Appl Environ Microbiol. 1982 Jan;43(1):57–64. doi: 10.1128/aem.43.1.57-64.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackie R. I., Bryant M. P. Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO(2) to Methanogenesis in Cattle Waste at 40 and 60 degrees C. Appl Environ Microbiol. 1981 Jun;41(6):1363–1373. doi: 10.1128/aem.41.6.1363-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]