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Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1987 Jul;53(7):1711–1713. doi: 10.1128/aem.53.7.1711-1713.1987

Appearance of enzyme activities catalyzing conversion of sterigmatocystin to aflatoxin B1 in late-growth-phase Aspergillus parasiticus cultures.

T E Cleveland 1, A R Lax 1, L S Lee 1, D Bhatnagar 1
PMCID: PMC203939  PMID: 3116930

Abstract

Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two activities into a postmicrosomal activity which catalyzed conversion of sterigmatocystin to O-methylsterigmatocystin and a microsomal activity which converted O-methylsterigmatocystin to aflatoxin B1. The two activities were absent in 24-h-old cells, increased to optimum levels during the stationary phase, and then declined.

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Selected References

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