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. 1987 Aug;53(8):1855–1859. doi: 10.1128/aem.53.8.1855-1859.1987

Isolation and characterization of mutants blocked in T-2 toxin biosynthesis.

M N Beremand 1
PMCID: PMC204013  PMID: 3310887

Abstract

Mutants of Fusarium sporotrichioides NRRL 3299 that were blocked or altered in the biosynthesis of the trichothecene T-2 toxin were generated by UV treatment and identified by a rapid screen in which monoclonal antibodies to T-2 were used. Three stable mutants were isolated and chemically characterized. Two mutants accumulated diacetoxyscirpenol, which suggests that they were defective in the step required for the addition of a hydroxyl group to the C-8 position in the trichothecene core structure. The third mutant appeared to be partially blocked at an early step or regulatory point in the pathway. This represents the first isolation of mutants in a trichothecene biosynthetic pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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